Effects of cinnamon nanoparticles produced with ultrasonic waves on growth performance, antioxidant status, and hemato-biochemical metabolites of broiler chickens.

A comprehensive experiment was carried out to evaluate the impact of cinnamon nanoparticles (CNPs) on growth performance and hemato-biochemical parameters in broiler chickens. A total of 500 one-day-old Ross 308 chicks were randomly distributed into five dietary treatment groups in a completely randomized design, with each treatment consisting of five replicates containing 20 birds per replicate. The experimental treatments included: A control group receiving a basal diet without supplementation, and four groups supplemented with 150, 300, 450, or 600 mg of CNPs per kg of feed. CNPs were synthesized using an ultrasonic-assisted method. Results demonstrated that dietary inclusion of CNPs at 300-600 mg/kg significantly enhanced average daily weight gain, reduced feed conversion ratio, and decreased mortality (P < 0.05), and with the most pronounced benefits observed at the 450 mg/kg level. Supplementation with CNPs also improved the antioxidant defense system, as indicated by elevated total antioxidant capacity, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities, along with a marked reduction in serum malondialdehyde (MDA) levels (P < 0.05). These effects were most prominent at 450 mg/kg. Furthermore, supplementation with 450 and 600 mg/kg of cinnamon nanoparticles (CNPs) significantly decreased red blood cell (RBC) count, hemoglobin concentration, hematocrit values, heterophil percentage, and cortisol levels, while significantly increasing lymphocyte percentage (P < 0.05).. Additionally, dietary CNPs led to a reduction in serum concentrations of cholesterol, triglycerides, low-density lipoprotein (LDL), uric acid, creatinine, and aspartate aminotransferase (AST). In conclusion, dietary inclusion of 450 mg/kg CNPs was found to optimally improve growth performance, enhance antioxidant capacity, and positively modulate hematological and biochemical profiles in broiler chickens.