Farag Soha A, Dosoky Waleed M, Moussa Abdelrahim A, Kamal Mahmoud, Elolimy Ahmed A, El-Mekkawy Mohamed M F, Abd El-Hack Mohamed E, Swelum Ayman A
Department of Animal Production, Faculty of Agriculture, Tanta University, Egypt.
Department of Animal and Fish Production, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.
Poult Sci. 2025 Jul;104(7):105164. doi: 10.1016/j.psj.2025.105164. Epub 2025 Apr 13.
This study aimed to evaluate the effects of date palm pollen (DPP) supplementation in the diet of Japanese quail on growth performance, carcass characteristics, blood biomarkers, and intestinal bacterial load. A total of 360 unsexed one-day-old Japanese quail chicks were randomly assigned to four experimental groups using a completely randomized design. Each group was further subdivided into three replicates, each consisting of 30 chicks. In the experiment, the first group was given control (basal diet); the second group was given the same basal diet plus 3 g/kg of DPP; the third group was given the same basal diet plus 5 g/kg of DPP; and the fourth group was given the same basal diet plus 7 g/kg of DPP. The results revealed significant differences between treatments, with DPP supplementation leading to increased body weight (BW) and body weight gain (BWG) during the initial weeks (P < 0.05), and these differences became more pronounced (P < 0.01) in the later stages of the study. Additionally, the DPP-treated groups demonstrated lower feed intake (FI) and improved feed conversion ratio (FCR) than the control group. Supplementation with DPP significantly (P < 0.05) influenced carcass, liver, spleen, thymus, and bursa percentage. The addition of DPP to the quail diet significantly impacted (P < 0.01) all hematological parameters, except for red blood cell concentration. Biochemical analysis showed a significant increase (P < 0.01) in total protein, albumin, globulin, and high-density lipoprotein (HDL) levels in the DPP groups. In contrast, alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, creatinine, cholesterol, triglycerides, and low-density lipoprotein (LDL) levels were significantly reduced (P < 0.01). DPP supplementation had a significant impact on antioxidant enzyme activities, with the 5 g/kg and 7 g/kg DPP groups showing significant increases (P < 0.01) in total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) levels. Furthermore, malondialdehyde (MDA) and nitric oxide (NO) concentrations were significantly reduced (P < 0.01). According to microbiological tests, the DPP-treated groups had reduced Escherichia coli and Staphylococcus aureus levels. In summary, adding DPP to the diet of Japanese quail enhances their gut microbiota composition, growth performance, carcass characteristics, and biochemical markers.
本研究旨在评估在日本鹌鹑日粮中添加枣椰花粉（DPP）对其生长性能、胴体特征、血液生物标志物和肠道细菌载量的影响。总共360只一日龄未分性别的日本鹌鹑雏鸡采用完全随机设计随机分为四个实验组。每组进一步细分为三个重复，每组由30只雏鸡组成。在实验中，第一组给予对照（基础日粮）；第二组给予相同的基础日粮加3 g/kg的DPP；第三组给予相同的基础日粮加5 g/kg的DPP；第四组给予相同的基础日粮加7 g/kg的DPP。结果显示各处理组之间存在显著差异，在最初几周添加DPP导致体重（BW）和体重增加（BWG）增加（P < 0.05），并且在研究后期这些差异变得更加明显（P < 0.01）。此外，与对照组相比，DPP处理组的采食量（FI）较低，饲料转化率（FCR）有所改善。添加DPP对胴体、肝脏、脾脏、胸腺和法氏囊百分比有显著影响（P < 0.05）。在鹌鹑日粮中添加DPP对所有血液学参数均有显著影响（P < 0.01），除了红细胞浓度。生化分析表明，DPP组的总蛋白、白蛋白、球蛋白和高密度脂蛋白（HDL）水平显著升高（P < 0.01）。相比之下，丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、尿素、肌酐、胆固醇、甘油三酯和低密度脂蛋白（LDL）水平显著降低（P < 0.01）。添加DPP对抗氧化酶活性有显著影响，5 g/kg和7 g/kg DPP组的总抗氧化能力（TAC）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）水平显著升高（P < 0.01）。此外，丙二醛（MDA）和一氧化氮（NO）浓度显著降低（P < 0.01）。根据微生物学检测，DPP处理组的大肠杆菌和金黄色葡萄球菌水平降低。总之，在日本鹌鹑日粮中添加DPP可改善其肠道微生物群组成、生长性能、胴体特征和生化指标。
