Yin Liyun, Liu Zhuomiao, Zhao Jian, Chen Shu, Wang Xiaochuan, Wang Zhenyu
College of Life Sciences, Hebei Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology, Hebei Collaborative Innovation Center for Eco-Environment, Hebei Normal University, Shijiazhuang 050024, China.
Institute of Coastal Environmental Pollution Control, Laboratory of Marine Environment, and Ecology and Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266100, China.
Eco Environ Health. 2025 Aug 5;4(3):100174. doi: 10.1016/j.eehl.2025.100174. eCollection 2025 Sep.
Engineered nanoparticles (ENPs) accumulate in marine sediments and exhibit adverse effects on benthic organisms. However, the effect of ENPs on marine benthic food chains is largely unknown. Herein, we investigated the trophic transfer and transformation of CeO ENPs within a simulated marine benthic food chain from clamworm () to turbot (), as well as their effects on fish flesh quality. The results showed that Ce contents in turbot increased with the accumulation of CeO ENPs in clamworm, but no biomagnification of CeO ENPs occurred along this food chain. During trophic transfer, CeO ENPs in turbot experienced transformation from Ce(IV) to Ce(III). Importantly, CeO ENPs accumulated in the muscle of turbot and decreased the crude protein, total amino acid, and delicious amino acid contents, as well as the texture of the muscle. CeO ENPs induced the deterioration of flesh quality, which was mainly related to metabolism in muscle and intestinal disorders caused by oxidative stress. Specifically, CeO ENPs increased the relative abundance of and in the turbot intestine while decreasing those of , , and . Significant disturbances in purine and amino acid (aspartate, glutamate, glycine, etc.) metabolism in muscle were induced by CeO ENPs. Moreover, correlation analysis showed that microbiota dysbiosis was highly correlated with muscle metabolic dysfunction. Our study provides insights into the transfer and transformation of CeO ENPs and their interference with fish flesh quality via the gut-muscle axis, providing useful information on assessing ecological risk and food safety in marine environments.
工程纳米颗粒（ENPs）在海洋沉积物中积累，并对底栖生物产生不利影响。然而，ENPs对海洋底栖食物链的影响在很大程度上尚不清楚。在此，我们研究了CeO ENPs在从沙蚕（）到大菱鲆（）的模拟海洋底栖食物链中的营养传递和转化，以及它们对鱼肉品质的影响。结果表明，随着沙蚕中CeO ENPs的积累，大菱鲆体内的Ce含量增加，但沿该食物链未发生CeO ENPs的生物放大作用。在营养传递过程中，大菱鲆体内的CeO ENPs经历了从Ce(IV)到Ce(III)的转化。重要的是，CeO ENPs在大菱鲆肌肉中积累，降低了粗蛋白、总氨基酸和呈味氨基酸含量以及肌肉质地。CeO ENPs导致鱼肉品质恶化，这主要与肌肉中的代谢以及氧化应激引起的肠道紊乱有关。具体而言，CeO ENPs增加了大菱鲆肠道中 和 的相对丰度，同时降低了 、 和 的相对丰度。CeO ENPs诱导了肌肉中嘌呤和氨基酸（天冬氨酸、谷氨酸、甘氨酸等）代谢的显著紊乱。此外，相关性分析表明微生物群失调与肌肉代谢功能障碍高度相关。我们的研究为CeO ENPs的转移和转化及其通过肠 - 肌轴对鱼肉品质的干扰提供了见解，为评估海洋环境中的生态风险和食品安全提供了有用信息。
