Li Linzi, Shah Amit, Ko Yi-An, Johnson Dayna A, Romaguera Dora, Alonso-Gómez Angel M, Toledo Estefanía, Razquin Cristina, Konieczna Jadwiga, Martinez-Gonzalez Miguel Angel, Warnberg Julia, Fitó Montserrat, Alonso Alvaro
Department of Epidemiology, Rollins School of Public Health, Emory University.
Department of Biostatistics and Bioinformatics, Rollins School of Public Health, Emory University.
medRxiv. 2025 Aug 28:2025.08.25.25334401. doi: 10.1101/2025.08.25.25334401.
Blood biomarkers can characterize the atrial substrate, helping to elucidate mechanisms of atrial fibrillation (AF) development. Understanding whether sedentary behavior affects AF-related biomarkers is key for future prevention strategies.
We studied 252 participants in PREDIMED-Plus, a multicenter randomized trial in Spain for the primary prevention of cardiovascular disease. A wrist-worn accelerometer was used to measure physical activity (PA) in a week at baseline and at least once during follow-up at years 3 and 5. Blood samples were collected at each time point to measure selected cardiovascular biomarkers: propeptide of procollagen type I, high-sensitivity (hs) troponin T (hsTnT), hs C-reactive protein, 3-nitrotyrosine, and N-terminal propeptide of B-type natriuretic peptide (NT-proBNP). Sedentary time was assessed as inactive time (< 1.5 METs in waking time). Using isotemporal substitution, we analyzed the impact of replacing 30-min sedentary time per day with low-intensity PA (LPA, 1.5-3 METs), moderate to vigorous PA (MVPA, > 3 METs) and time in bed (time difference between going to bed and getting up) on log-transformed biomarkers cross-sectionally and longitudinally, using linear regression and mixed models.
At baseline, 252 eligible participants averaged 65 years of age (SD 4.9), and BMI 32.2 kg/m (SD 3.3), and 40% were female. Cross-sectionally, replacing 30-min sedentary time with LPA, MVPA, or time in bed had no significant association with biomarkers. After 5 years, replacing baseline 30-min of sedentary time per day with LPA or MVPA was associated with lower hs-TnT concentrations compared to baseline (-4%, 95% CI -8%, -1%; -2%, 95% CI -7%, 4%, respectively). Substituting 30-min sedentary time with LPA or MVPA showed nonsignificant reductions in NT-proBNP (-3%, 95% CI -11%, 5%; -8%, 95% CI -20%, 5%, respectively), but not a consistent association with other biomarkers.
In overweight/obese individuals, prolonged sedentary time, when compared to engaging in PA, was associated with unfavorable changes of hs-TnT over 5 years, but no significant impact on other AF-related biomarkers.
血液生物标志物可表征心房基质，有助于阐明心房颤动（AF）的发生机制。了解久坐行为是否会影响与AF相关的生物标志物是未来预防策略的关键。
我们研究了PREDIMED-Plus研究中的252名参与者，这是一项在西班牙进行的多中心随机试验，用于心血管疾病的一级预防。使用腕部佩戴的加速度计在基线时测量一周的身体活动（PA），并在第3年和第5年的随访期间至少测量一次。在每个时间点采集血样，以测量选定的心血管生物标志物：I型前胶原原肽、高敏（hs）肌钙蛋白T（hsTnT）、hs C反应蛋白、3-硝基酪氨酸和B型利钠肽（NT-proBNP）的N端前肽。久坐时间被评估为非活动时间（清醒时间<1.5代谢当量）。使用等时替代法，我们通过线性回归和混合模型，分析了用低强度PA（LPA，1.5-3代谢当量）、中度至剧烈PA（MVPA，>3代谢当量）和卧床时间（上床睡觉和起床之间的时间差）替代每天30分钟久坐时间对经对数转换的生物标志物的横断面和纵向影响。
在基线时，252名符合条件的参与者平均年龄为65岁（标准差4.9），BMI为32.2 kg/m²（标准差3.3），40%为女性。横断面分析显示，用LPA、MVPA或卧床时间替代30分钟久坐时间与生物标志物无显著关联。5年后，与基线相比，用LPA或MVPA替代每天基线的30分钟久坐时间与较低的hs-TnT浓度相关（分别为-4%，95%CI -8%，-1%；-2%，95%CI -7%，4%）。用LPA或MVPA替代30分钟久坐时间显示NT-proBNP有非显著降低（分别为-3%，95%CI -11%，5%；-8%，95%CI -20%，5%），但与其他生物标志物没有一致的关联。
在超重/肥胖个体中，与进行身体活动相比，长期久坐与5年内hs-TnT的不良变化有关，但对其他与AF相关的生物标志物没有显著影响。
