Yamashita Mitsuaki, Saneoka Fuki, Sano Aozora, Arakawa Towa, Iida Akira
Graduate School of Agriculture, Kindai University.
J Pestic Sci. 2025 Aug 20;50(3):64-73. doi: 10.1584/jpestics.D25-026.
This study focused on the chemical synthesis of auxin analogs, wherein a trifluoromethyl group was introduced near the carboxyl group in the side chain of natural and synthetic auxins, including IAA, NAA, IBA, 2,4-D, and 4-Cl-IAA. The effects of these synthetic compounds and natural auxins on plant growth regulation and callus growth were evaluated. In experiments with black gram, CF-IAA and 4-Cl-CF-IAA exhibited comparable effects to the parent compound, IAA. Meanwhile, CF-NAA, CF-2,4-D, CF-IBA-1, and CF-IBA-2 displayed effects that differed considerably from those of their respective parent auxins. In experiments with lettuce, CF-IAA, 4-Cl-CF-IAA, CF-NAA, CF-2,4-D, and CF-IBA-1 showed effects comparable to the corresponding parent auxins. However, at low concentrations, these analogs induced hypocotyl and root elongations, a response distinct from that observed with their parent compounds. Furthermore, CF-IBA-2 considerably promoted hypocotyl and root elongations across all concentrations relative to the control. The addition of synthetic compounds to callus cultures revealed that CF-IAA, 4-Cl-CF-IAA, CF-NAA, and CF-2,4-D promoted callus proliferation, whereas CF-IBA-1 and CF-IBA-2 did not enhance callus growth.
本研究聚焦于生长素类似物的化学合成，即在天然和合成生长素（包括吲哚 - 3 - 乙酸（IAA）、萘乙酸（NAA）、吲哚 - 3 - 丁酸（IBA）、2,4 - 二氯苯氧乙酸（2,4 - D）和4 - 氯吲哚 - 3 - 乙酸（4 - Cl - IAA））的侧链羧基附近引入三氟甲基基团。评估了这些合成化合物和天然生长素对植物生长调节和愈伤组织生长的影响。在对黑绿豆的实验中，CF - IAA和4 - Cl - CF - IAA表现出与母体化合物IAA相当的效果。同时，CF - NAA、CF - 2,4 - D、CF - IBA - 1和CF - IBA - 2显示出与其各自母体生长素显著不同的效果。在对生菜的实验中，CF - IAA、4 - Cl - CF - IAA、CF - NAA、CF - 2,4 - D和CF - IBA - 1表现出与相应母体生长素相当的效果。然而，在低浓度下，这些类似物诱导下胚轴和根伸长，这一反应与其母体化合物所观察到的不同。此外，相对于对照，CF - IBA - 2在所有浓度下都显著促进下胚轴和根伸长。将合成化合物添加到愈伤组织培养物中发现，CF - IAA、4 - Cl - CF - IAA、CF - NAA和CF - 2,4 - D促进愈伤组织增殖，而CF - IBA - 1和CF - IBA - 2并未促进愈伤组织生长。
