He Qin-Yang, Peng Jie, Yu Ruo-Xing, Luo Ting, Li Yao-Hui, Zhong Zi-Rou, Qin Zi-Le, Wang Fei, Li Jian, Luo Sheng-Wei
Engineering Research Center of Polyploidy fish Reproduction and Breeding of the State Education Ministry, College of Life Science, Hunan Normal University, Changsha, 410081, People's Republic of China.
Mar Biotechnol (NY). 2025 Sep 5;27(5):133. doi: 10.1007/s10126-025-10513-1.
Triploid cyprinid fish (TCF, 3N = 150) is a novel hybrid fish showing great disease resistance during aquaculture processes. However, the majority of Aeromonas strains act as opportunistic pathogens that can cause a variety of diseases and pose a notable health risk. In this investigation, a novel Aeromonas sp. AS1-4 was retrieved from the spleen (SPL) of moribund cyprinid fish. Whole genome sequencing (WGS) was employed to characterize its virulence features and signal regulation. Thereafter, TCFs were infected with strain AS1-4 for pathological analysis. Strain AS1-4 challenge could cause abnormal glycogen deposition in the liver (LIV) of TCFs, accompanied by significant alteration of physiological response. Furthermore, expression patterns of pivotal immune-related genes were markedly elevated in TCFs post-infection. Transcriptomic analysis revealed that strain AS1-4 infection may exert a profound impact on insulin signaling pathway and steroid biosynthesis. These results may contribute to the comprehension of molecular characteristics in fish infected with Aeromonas sp. AS1-4.
三倍体鲤科鱼类（TCF，3N = 150）是一种新型杂交鱼，在水产养殖过程中表现出很强的抗病能力。然而，大多数气单胞菌菌株作为机会致病菌，可导致多种疾病，并构成显著的健康风险。在本研究中，从濒死鲤科鱼类的脾脏（SPL）中分离出一种新型气单胞菌AS1-4。采用全基因组测序（WGS）来表征其毒力特征和信号调控。此后，用AS1-4菌株感染TCF进行病理分析。AS1-4菌株攻击可导致TCF肝脏（LIV）中糖原异常沉积，并伴有生理反应的显著改变。此外，感染后TCF中关键免疫相关基因的表达模式显著升高。转录组分析表明，AS1-4菌株感染可能对胰岛素信号通路和类固醇生物合成产生深远影响。这些结果可能有助于理解感染气单胞菌AS1-4的鱼类的分子特征。
