拟南芥中钙激活植物激酶的差异调控

Differential regulation of calcium-activated plant kinases in Arabidopsis thaliana.

作者信息

Zauser Martin, Liese Anja, Feldman-Salit Anna, Krebs Melanie, Schumacher Karin, Romeis Tina, Kummer Ursula, Pahle Jürgen

机构信息

Biological Information Processing Group, BioQuant, Heidelberg University, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany.

Department of Plant Biochemistry of Interactions, Leibniz Institute of Plant Biochemistry (IPB), Halle, Germany.

出版信息

Plant J. 2025 Sep;123(5):e70413. doi: 10.1111/tpj.70413.

DOI:10.1111/tpj.70413

PMID:40911494

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12412779/

Abstract

The decoding of calcium signals by plant calcium-dependent kinases (CPKs) is not fully understood yet. Based on kinetic in vitro measurements of the activity of several CPK proteins, their individual activity profile was modeled and coupled to cytosolic calcium concentration changes from in vivo measurements of guard cells and epidermal leaf cells. In addition, computationally produced surrogate data were used. It was analyzed how individual activity profiles of CPKs will change in response to the different calcium time courses. In addition, the impact of modeling explicitly individual elementary reaction steps for binding and unbinding in comparison to using a heuristic Hill equation for the binding process was investigated.

摘要

植物钙依赖蛋白激酶（CPK）对钙信号的解码尚未完全明晰。基于对几种CPK蛋白活性的体外动力学测量，模拟了它们各自的活性曲线，并将其与保卫细胞和叶片表皮细胞体内测量得到的胞质钙浓度变化相耦合。此外，还使用了通过计算生成的替代数据。分析了CPK的个体活性曲线如何响应不同的钙时间进程而变化。此外，还研究了与使用关于结合过程的启发式希尔方程相比，对结合和解离的各个基本反应步骤进行显式建模的影响。