Rennich Emily, Sung Suk Hyun, Agarwal Nishkarsh, Gates Maya, Kerns Robert, Hovden Robert, Baggari Ismail El
The Rowland Institute at Harvard, Harvard University, Cambridge, MA 02138.
Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109.
Proc Natl Acad Sci U S A. 2025 Sep 9;122(36):e2509736122. doi: 10.1073/pnas.2509736122. Epub 2025 Sep 5.
Cryogenic transmission electron microscopy has revolutionized structural biology and materials science. To image below liquid nitrogen temperatures, various liquid helium stages have been constructed but have proven to be complex and unstable, making high-resolution imaging challenging. This problem is even more pronounced in side-entry specimen holders common on modern transmission electron microscopes. Here, we introduce an ultracold liquid helium transmission electron microscope side-entry specimen holder, featuring continuous cryogen flow and vibration decoupling. This instrument is compatible with modern aberration-corrected microscopes and achieves sub-25 K base temperature, [Formula: see text]2 mK thermal stability over many hours, and atomic resolution-setting the stage for a new era of cryogenic electron microscopy.
低温透射电子显微镜彻底改变了结构生物学和材料科学。为了在液氮温度以下成像，人们构建了各种液氦阶段，但事实证明它们复杂且不稳定，使得高分辨率成像具有挑战性。这个问题在现代透射电子显微镜常见的侧入式样品架中更为突出。在这里，我们介绍一种超冷液氦透射电子显微镜侧入式样品架，其特点是有连续的低温制冷剂流动和振动去耦。该仪器与现代像差校正显微镜兼容，实现了低于25 K的基础温度、数小时内±2 mK的热稳定性以及原子分辨率，为低温电子显微镜的新时代奠定了基础。
