Failler J M, Farinotti R, Dauphin A
Ann Biol Clin (Paris). 1985;43(4):341-5.
Fluorescence polarization immunoassays (FPIA) of aminoglycoside antibiotics (gentamicin, tobramycin and amikacin) in plasma have been described. The use of sisomicin, a structural analogue of a C1a gentamicin fraction, is an alternative to gentamicin therapy for economical reasons. We have tested a therapeutic monitoring of this drug bases on FPIA using its croos reaction with gentamicin. Tracer (gentamicin labelled with fluorescein) and antisera were purchased from Abbott S.A. (gentamicin kit). Standard curves were obtained with sisomicin standard plasma samples. Within run and run-to-run precisions expressed as coefficient of variation were lower than 9.2 p. cent. The measurement of concentrations as low as 0.15 mg/l are possible. In order to evaluate specificity of this assay in clinical situation, we compared FPIA and liquid chromatography results.
已报道了血浆中氨基糖苷类抗生素（庆大霉素、妥布霉素和阿米卡星）的荧光偏振免疫分析（FPIA）。由于经济原因，使用西索米星（一种C1a庆大霉素组分的结构类似物）作为庆大霉素治疗的替代药物。我们基于FPIA，利用其与庆大霉素的交叉反应，对该药物进行了治疗监测。示踪剂（用荧光素标记的庆大霉素）和抗血清购自雅培公司（庆大霉素试剂盒）。用西索米星标准血浆样本获得标准曲线。以变异系数表示的批内和批间精密度低于9.2%。能够测量低至0.15 mg/l的浓度。为了评估该分析方法在临床情况下的特异性，我们比较了FPIA和液相色谱的结果。
