Tod M, Resplandy G, Farinotti R, Provost Y, Dauphin A
Laboratoire de Toxicologie et de Dosage des Médicaments, C.H.U., Bichat, Paris, France.
J Pharm Biomed Anal. 1990;8(3):279-82. doi: 10.1016/0731-7085(90)80037-p.
Hydroquinidine is a structural analogue of quinidine. It is used in the treatment and prevention of cardiac arrhythmias and necessitates serum monitoring. Fluorescence polarization immunoassay (FPIA) of quinidine has been proposed and we have tested the performance of this assay for hydroquinidine using its cross-reaction with quinidine. Tracer (quinidine labelled with fluorescein) and anti-serum were purchased from Abbott S.A. Standard curves were obtained using specifically prepared hydroquinidine calibrators and within-run and run-to-run precision values (expressed as relative standard deviation) (RSD) lower than 5.3% (n = 10). In order to evaluate specificity of this assay in the clinical situation, FPIA and liquid chromatography results were compared.
氢化奎尼丁是奎尼丁的结构类似物。它用于治疗和预防心律失常，需要进行血清监测。已有人提出奎尼丁的荧光偏振免疫测定法（FPIA），我们利用氢化奎尼丁与奎尼丁的交叉反应测试了该方法对氢化奎尼丁的检测性能。示踪剂（用荧光素标记的奎尼丁）和抗血清购自雅培公司。使用专门制备的氢化奎尼丁校准品获得标准曲线，批内和批间精密度值（以相对标准偏差表示）（RSD）低于5.3%（n = 10）。为了评估该方法在临床情况下的特异性，对FPIA和液相色谱结果进行了比较。
