牛的多组织单细胞表达图谱。

A multi-tissue single-cell expression atlas in cattle.

作者信息

Han Bo, Li Houcheng, Zheng Weijie, Zhang Qi, Chen Ao, Zhu Senlin, Shi Tao, Wang Fei, Zou Dong, Song Yu, Ye Wen, Du Aixia, Fu Yihan, Jia Minghui, Bai Zhonghao, Yuan Zhixiang, Liu Wansheng, Tuo Wenbin, Hope Jayne C, MacHugh David E, O'Grady John F, Madsen Ole, Sahana Goutam, Luo Yonglun, Lin Lin, Li Congjun, Cai Zexi, Li Bingjie, Huang Jinming, Liu Lin, Zhang Zhang, Ma Zhu, Hou Yali, Liu George E, Jiang Yu, Sun Hui-Zeng, Fang Lingzhao, Sun Dongxiao

机构信息

State Key Laboratory of Animal Biotech Breeding, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, China Agricultural University, Beijing, China.

Center for Quantitative Genetics and Genomics, Aarhus University, Aarhus, Denmark.

出版信息

Nat Genet. 2025 Sep 5. doi: 10.1038/s41588-025-02329-5.

DOI:10.1038/s41588-025-02329-5

PMID:40913183

Abstract

Systematic characterization of the molecular states of cells in livestock tissues is essential for understanding the cellular and genetic mechanisms underlying economically and ecologically important physiological traits. Here, as part of the Farm Animal Genotype-Tissue Expression (FarmGTEx) project, we describe a comprehensive reference map including 1,793,854 cells from 59 bovine tissues in calves and adult cattle, spanning both sexes, which reveals intra-tissue and inter-tissue cellular heterogeneity in gene expression, transcription factor regulation and intercellular communication. Integrative analysis with genetic variants that underpin bovine monogenic and complex traits uncovers cell types of relevance, such as spermatocytes, responsible for sperm motility and excitatory neurons for milk fat yield. Comparative analysis reveals similarities in gene expression between cattle and humans, allowing for the detection of relevant cell types to study human complex phenotypes. This Cattle Cell Atlas will serve as a key resource for cattle genetics and genomics, selective breeding and comparative biology.

摘要

系统表征家畜组织中细胞的分子状态对于理解经济和生态上重要生理性状背后的细胞和遗传机制至关重要。在此，作为农场动物基因型-组织表达（FarmGTEx）项目的一部分，我们描述了一个全面的参考图谱，其中包括来自犊牛和成年牛59个组织的1,793,854个细胞，涵盖两性，揭示了组织内和组织间在基因表达、转录因子调控和细胞间通讯方面的细胞异质性。与支撑牛单基因和复杂性状的遗传变异进行综合分析，揭示了相关的细胞类型，如负责精子活力的精母细胞和影响乳脂产量的兴奋性神经元。比较分析揭示了牛和人类之间基因表达的相似性，有助于检测与人类复杂表型相关的细胞类型。这个牛细胞图谱将成为牛遗传学和基因组学、选择性育种和比较生物学的关键资源。