一种用于检测软骨中活性连接蛋白的灵敏测定法。
A sensitive assay for active link protein from cartilage.
作者信息
Sandy J D, Plaas A H
出版信息
Biochem J. 1985 Dec 1;232(2):613-6. doi: 10.1042/bj2320613.
Abstract
A new assay for the activity of cartilage link protein is described. The method is based on the finding [Plaas, Sandy & Muir (1983) Biochem. J. 214, 855-864] that addition of link protein to [35S]sulphate-labelled proteoglycan aggregates from rabbit chondrocyte cultures resulted in the formation of link-stabilized aggregates. The percentage aggregate was found to be related linearly to the amount of purified bovine link protein added in the 20-120 ng range. The assay was used to monitor loss of link-protein activity during heat denaturation and to measure binding of link protein by purified proteoglycan monomer.
摘要
本文描述了一种用于检测软骨连接蛋白活性的新方法。该方法基于以下发现[普拉斯、桑迪和缪尔（1983年），《生物化学杂志》214卷，第855 - 864页]：向来自兔软骨细胞培养物的[35S]硫酸盐标记的蛋白聚糖聚集体中添加连接蛋白会导致形成连接稳定的聚集体。发现在20 - 120纳克范围内，聚集体的百分比与添加的纯化牛连接蛋白的量呈线性关系。该检测方法用于监测热变性过程中连接蛋白活性的丧失，并测量纯化的蛋白聚糖单体与连接蛋白的结合。
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A sensitive assay for active link protein from cartilage.一种用于检测软骨中活性连接蛋白的灵敏测定法。
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本文引用的文献
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A comparative study of the binding of cartilage link protein and the hyaluronate-binding region of the cartilage proteoglycan to hyaluronate-substituted Sepharose gel.软骨连接蛋白及软骨蛋白聚糖的透明质酸结合区与透明质酸取代的琼脂糖凝胶结合的比较研究。
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Equilibrium and kinetic studies of the interaction between cartilage proteoglycan and hyaluronate.软骨蛋白聚糖与透明质酸相互作用的平衡及动力学研究。
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Enzyme-linked immunosorbent assay analyses of the hyaluronate-binding region and the link protein of proteoglycan aggregate.蛋白聚糖聚集体的透明质酸结合区域和连接蛋白的酶联免疫吸附测定分析
J Biol Chem. 1982 Dec 10;257(23):14173-80.
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Physical properties of the hyaluronate binding region of proteoglycan from pig laryngeal cartilage. Densitometric and small-angle neutron scattering studies of carbohydrates and carbohydrate-protein macromolecules.猪喉软骨蛋白聚糖透明质酸结合区域的物理性质。碳水化合物及碳水化合物-蛋白质大分子的光密度测定和小角中子散射研究。
J Mol Biol. 1981 Jul 25;150(1):69-95. doi: 10.1016/0022-2836(81)90325-9.
7
Proteoglycans from bovine nasal cartilage. Immunochemical studies of link protein.来自牛鼻软骨的蛋白聚糖。连接蛋白的免疫化学研究。
J Biol Chem. 1980 Oct 10;255(19):9295-305.
8
Age-related decrease in the link-stability of proteoglycan aggregates formed by articular chondrocytes.关节软骨细胞形成的蛋白聚糖聚集体的连接稳定性随年龄增长而降低。
Biochem J. 1984 May 15;220(1):337-40. doi: 10.1042/bj2200337.
9
Proteoglycan aggregate formation by articular chondrocytes. Decrease in link-protein synthesis during culture.关节软骨细胞形成蛋白聚糖聚集体。培养过程中连接蛋白合成减少。
Biochem J. 1983 Sep 15;214(3):855-64. doi: 10.1042/bj2140855.
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Age-related changes in the structure of proteoglycan link proteins present in normal human articular cartilage.正常人体关节软骨中蛋白聚糖连接蛋白结构的年龄相关变化。
Biochem J. 1983 Jul 15;214(1):269-72. doi: 10.1042/bj2140269.
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