关节软骨细胞形成的蛋白聚糖聚集体的连接稳定性随年龄增长而降低。
Age-related decrease in the link-stability of proteoglycan aggregates formed by articular chondrocytes.
作者信息
Plaas A H, Sandy J D
出版信息
Biochem J. 1984 May 15;220(1):337-40. doi: 10.1042/bj2200337.
Abstract
Chondrocytes were isolated from the articular cartilage of rabbits aged between 6 and 50 weeks and labelled with [35S]sulphate after 48 h in monolayer culture. The percentage of the total proteoglycan monomers synthesized by each culture that were present as link-stabilized aggregates was shown to be about 83% at 6, 9 and 12 weeks, 73% at 15 weeks, 48% at 30 weeks and 32% at 50 weeks. The proliferative activity of the cells in culture also decreased markedly with the age of the donor. The results suggest that aging of chondrocytes in vivo is accompanied by a decrease in their capacity for link-protein synthesis.
摘要
从6至50周龄兔子的关节软骨中分离出软骨细胞，在单层培养48小时后用[35S]硫酸盐进行标记。在6周、9周和12周时，每种培养物合成的作为连接稳定聚集体存在的蛋白聚糖单体总量的百分比约为83%，15周时为73%，30周时为48%，50周时为32%。培养细胞的增殖活性也随着供体年龄的增长而显著降低。结果表明，体内软骨细胞的老化伴随着其连接蛋白合成能力的下降。
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