使用RGD和EC的瓣膜材料的生物学特性。
Biological properties of valve materials using RGD and EC.
作者信息
Xu Chennian, Liu Yang, Qiao Rui, Jin Ping, Tang Hao, Tian Zhiyuan, Cui Bin, Wen Anguo, Yang Jian
机构信息
Key Laboratory of Gastrointestinal Pharmacology of Chinese Materia Medica of the State Administration of Traditional Chinese Medicine, Department of Pharmacology, School of Pharmacy, The Fourth Military Medical University, Xi'an, 710032, Shaanxi, China.
Department of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, 710032, Shaanxi, P. R. China.
出版信息
Open Med (Wars). 2025 Sep 4;20(1):20251195. doi: 10.1515/med-2025-1195. eCollection 2025.
BACKGROUND
Rapid application of transcatheter valve replacement for valve diseases has promoted the development of biological valves. Different efforts have been made to improve the surface properties of valves. We developed a new method using arginine-glycine-aspartate (RGD) peptide and epoxy chloropropane (EC). Our goal was to evaluate and detect the surface biological characteristics of valve materials using RGD and EC treatments.
METHODS
The surfaces of the valve materials used in the GA-EC and RGD-EC groups were smooth and relatively dense, as observed using a scanning electron microscope.
RESULTS
More MSCs adhered to and grew on the samples in the GA-EC and RGD-EC groups than in the GA group. The apoptosis rate of MSCs was markedly decreased, whereas the expression of vimentin was elevated in the GA-EC and RGD-EC groups ( < 0.05). The adherent ability of MSCs in the RGD-EC and GA-EC groups was significantly higher than that in the GA group ( < 0.05).
CONCLUSIONS
The new treatment method using RGD and EC improves the biological properties of the surface of the biological valve materials.
背景
经导管瓣膜置换术在瓣膜疾病中的快速应用推动了生物瓣膜的发展。人们为改善瓣膜的表面特性做出了不同努力。我们开发了一种使用精氨酸 - 甘氨酸 - 天冬氨酸（RGD）肽和环氧氯丙烷（EC）的新方法。我们的目标是使用RGD和EC处理来评估和检测瓣膜材料的表面生物学特性。
方法
使用扫描电子显微镜观察，GA - EC组和RGD - EC组所用瓣膜材料的表面光滑且相对致密。
结果
与GA组相比，GA - EC组和RGD - EC组的样本上黏附并生长的间充质干细胞（MSC）更多。GA - EC组和RGD - EC组中MSC的凋亡率显著降低，而波形蛋白的表达升高（<0.05）。RGD - EC组和GA - EC组中MSC的黏附能力显著高于GA组（<0.05）。
结论
使用RGD和EC的新处理方法改善了生物瓣膜材料表面的生物学特性。
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