分离的大鼠肝细胞对转铁蛋白和铁的细胞内加工过程。
Intracellular processing of transferrin and iron by isolated rat hepatocytes.
作者信息
Young S P, Roberts S, Bomford A
出版信息
Biochem J. 1985 Dec 15;232(3):819-23. doi: 10.1042/bj2320819.
Abstract
Transferrin bound by isolated rat hepatocytes is rapidly endocytosed and enters a compartment of low density. Little was found associated with the lysosomes, even though the protein was subsequently lost from the cells. Iron entering the cells on transferrin was subsequently found in a number of intracellular components: transferrin, haem, ferritin and a residual fraction. After 2 h incubation with 59Fe-transferrin almost 70% of the iron was in ferritin, and this proportion increased to 80% during a 'chase' experiment. Residual iron, because of its rapid increase at the start of the incubation and its decline during the 'chase', probably represents an intracellular transit pool, which at steady state was present at 23 pg/10(6) cells.
摘要
被分离的大鼠肝细胞结合的转铁蛋白迅速被内吞，并进入低密度区室。即使随后蛋白质从细胞中丢失，也很少发现其与溶酶体相关。随后发现转铁蛋白携带进入细胞的铁存在于多种细胞内成分中：转铁蛋白、血红素、铁蛋白和一个残留部分。在用59Fe - 转铁蛋白孵育2小时后，近70%的铁存在于铁蛋白中，并且在“追踪”实验期间这一比例增加到80%。残留铁由于在孵育开始时迅速增加并在“追踪”期间下降，可能代表一个细胞内转运池，在稳态下其含量为23 pg/10(6)个细胞。
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