分离的大鼠肝细胞对转铁蛋白和铁的细胞内加工过程。
Intracellular processing of transferrin and iron by isolated rat hepatocytes.
作者信息
Young S P, Roberts S, Bomford A
出版信息
Biochem J. 1985 Dec 15;232(3):819-23. doi: 10.1042/bj2320819.
Abstract
Transferrin bound by isolated rat hepatocytes is rapidly endocytosed and enters a compartment of low density. Little was found associated with the lysosomes, even though the protein was subsequently lost from the cells. Iron entering the cells on transferrin was subsequently found in a number of intracellular components: transferrin, haem, ferritin and a residual fraction. After 2 h incubation with 59Fe-transferrin almost 70% of the iron was in ferritin, and this proportion increased to 80% during a 'chase' experiment. Residual iron, because of its rapid increase at the start of the incubation and its decline during the 'chase', probably represents an intracellular transit pool, which at steady state was present at 23 pg/10(6) cells.
摘要
被分离的大鼠肝细胞结合的转铁蛋白迅速被内吞，并进入低密度区室。即使随后蛋白质从细胞中丢失，也很少发现其与溶酶体相关。随后发现转铁蛋白携带进入细胞的铁存在于多种细胞内成分中：转铁蛋白、血红素、铁蛋白和一个残留部分。在用59Fe - 转铁蛋白孵育2小时后，近70%的铁存在于铁蛋白中，并且在“追踪”实验期间这一比例增加到80%。残留铁由于在孵育开始时迅速增加并在“追踪”期间下降，可能代表一个细胞内转运池，在稳态下其含量为23 pg/10(6)个细胞。
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本文引用的文献
1
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Biochim Biophys Acta. 1959 Oct;35:543. doi: 10.1016/0006-3002(59)90407-x.
2
The quantitative determination of iron kinetics and hemoglobin synthesis in human subjects.人体中铁动力学和血红蛋白合成的定量测定。
J Clin Invest. 1961 May;40(5):753-82. doi: 10.1172/JCI104310.
3
One-step growth curve of Western equine encephalomyelitis virus on chicken embryo cells grown in vitro and analysis of virus yields from single cells.西部马脑炎病毒在体外培养的鸡胚细胞上的一步生长曲线及单细胞病毒产量分析
J Exp Med. 1954 Feb;99(2):183-99. doi: 10.1084/jem.99.2.183.
4
The interaction of transferrin with isolated hepatocytes.转铁蛋白与分离的肝细胞之间的相互作用。
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5
Isolation and culture of Kupffer cells and hepatocytes from single rat livers: with observations on iron-loaded kupffer cells.
Lab Invest. 1981 Dec;45(6):558-66.
6
7
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8
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Anal Biochem. 1982 Mar 1;120(2):235-42. doi: 10.1016/0003-2697(82)90341-4.
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Br J Haematol. 1983 Sep;55(1):93-101. doi: 10.1111/j.1365-2141.1983.tb01227.x.
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