Boran Filiz, Koytepe Suleyman, Aslan Gizem, Birhanli Emre, Yesilada Ozfer, Alagoz Mehmet Abdullah
Department of Biology, Arts and Science Faculty, Inonu University, 44280 Malatya, Turkey.
Department of Chemistry, Arts and Science Faculty, Inonu University, 44280 Malatya, Turkey.
ACS Omega. 2025 Aug 22;10(34):38440-38451. doi: 10.1021/acsomega.5c01126. eCollection 2025 Sep 2.
Bacterial cellulose (BC) was produced in dried apricot extract medium (DAEM) by . The BC yield obtained from DAEM containing 0.5 g of glucose after 10 days of incubation at 30 °C was determined as 9.67 g/L. BC was used as an immobilization matrix for . First, structural characterizations of BC and BC-yeast were carried out. Also, their surface and morphological properties were examined by SEM and atomic force microscopy. Yeast attachment and growth kinetics were also evaluated experimentally and theoretically. This yeast-immobilized BC (BC-yeast) membrane was used for removal of textile dye, Reactive Blue 171 (RB 171). The results showed that yeast was successfully immobilized on BC and could be effectively used for dye removal. When 12 lyophilized BC-yeast samples were used, 52% and 21% dye removal values were obtained under static and shaking (150 rpm) conditions, respectively. These values were 49% and 50% for wet BC-yeast samples after 24 h, respectively. Additionally, density functional theory calculations were performed to elucidate the interactions responsible for the adsorption of RB 171 dye onto the BC structure, and it was shown that the RB 171 structure is energetically very strong and favorably bound to the BC surface. It was found that BC-yeast was highly effective for RB 171 removal. Therefore, the BC produced in DAEM could be used as an immobilization matrix and as a low-cost, effective, and alternative adsorbent for the removal of dyes from dye-containing wastewaters.
细菌纤维素（BC）由……在干杏提取物培养基（DAEM）中产生。在30℃孵育10天后，从含有0.5g葡萄糖的DAEM中获得的BC产量测定为9.67g/L。BC被用作……的固定化基质。首先，对BC和BC-酵母进行了结构表征。此外，通过扫描电子显微镜（SEM）和原子力显微镜检查了它们的表面和形态特性。还通过实验和理论评估了酵母的附着和生长动力学。这种固定化酵母的BC（BC-酵母）膜用于去除纺织染料活性蓝171（RB 171）。结果表明，酵母成功固定在BC上，并可有效地用于染料去除。当使用12个冻干的BC-酵母样品时，在静态和振荡（150rpm）条件下分别获得了52%和21%的染料去除率。24小时后，湿BC-酵母样品的这些值分别为49%和50%。此外，进行了密度泛函理论计算，以阐明负责RB 171染料吸附到BC结构上的相互作用，结果表明RB 171结构在能量上非常稳定，并与BC表面形成有利的结合。发现BC-酵母对RB 171的去除非常有效。因此，在DAEM中产生的BC可作为固定化基质，以及作为一种低成本、有效且可替代的吸附剂，用于从含染料废水中去除染料。
