Department of Biotechnology, KLE Technological University, Hubballi, Karnataka, 580031, India.
Department of Biotechnology, KLE Technological University, Hubballi, Karnataka, 580031, India.
Carbohydr Res. 2020 May;491:107979. doi: 10.1016/j.carres.2020.107979. Epub 2020 Mar 7.
Bacterial cellulose (BC) is an excellent natural biopolymer with wide range of applications. The present study reports a potential BC producing thermophile, identified as Bacillus licheniformis strain ZBT2. The thermophile produced pellicle form of BC (3.0 g/l) under static conditions. Statistical optimization of BC was carried out by Plackett-Burman and central composite design. Results suggest that BC yield (9.2 g/l) was enhanced with 6.6-fold after optimization. BC-gelatin hydrogels composites were developed to assess various properties. The water retention capability and moisture content properties of BC and composites were promising and also exhibited negligible protein adsorption. The composites also demonstrated to be consistent during controlled drug delivery profiling. Furthermore, the composites also demonstrated antibacterial efficiency against Escherichia coli and Micrococcus luteus. The structural, morphological and thermal properties were assessed by analytical techniques such as, fourier transform infrared spectroscopy, scanning electron microscopy with energy dispersive X-ray analysis, thermogravimetric analysis and differential scanning calorimetry analysis. The study reflects the exploitation of a thermophile for development of BC which can be a preferred choice as a scaffold for tissue engineering and drug-delivery systems.
细菌纤维素 (BC) 是一种具有广泛应用的优秀天然生物聚合物。本研究报告了一种潜在的产 BC 的嗜热菌，鉴定为地衣芽孢杆菌 ZBT2 菌株。该嗜热菌在静态条件下产生了薄膜状的 BC（3.0 g/l）。通过 Plackett-Burman 和中心复合设计对 BC 进行了统计优化。结果表明，优化后 BC 的产量（9.2 g/l）提高了 6.6 倍。开发了 BC-明胶水凝胶复合材料来评估各种性能。BC 和复合材料的保水能力和水分含量特性很有前景，并且对蛋白质吸附也可以忽略不计。复合材料在控制药物释放分析中也表现出一致性。此外，该复合材料还表现出对大肠杆菌和藤黄微球菌的抗菌效率。通过傅里叶变换红外光谱、带有能量色散 X 射线分析的扫描电子显微镜、热重分析和差示扫描量热分析等分析技术评估了复合材料的结构、形态和热性能。该研究反映了利用嗜热菌开发 BC 的情况，BC 可以作为组织工程和药物输送系统的支架的首选。
