Chen Jiajing, Wu Xiaodan, Wang Xintong, Yuan Ruijia, Zhu Qi
Emergency Department, Central Hospital Affiliated to Shenyang Medical College, Shenyang, China.
School of Clinical Medicine, China Medical University, Shenyang, China.
Front Microbiol. 2025 Aug 22;16:1580231. doi: 10.3389/fmicb.2025.1580231. eCollection 2025.
Acute stroke (AS) is a major public health issue globally, exhibiting high morbidity, disability rate, and mortality. Emerging research has demonstrated the critical roles of gut microbiota and its metabolites in pathogenesis, recovery, and prognosis of AS.
In this study, we investigated alterations in gut microbiota composition and metabolomic profiles in AS patients using 16S rRNA sequencing and untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics technology.
The results revealed significant changes in gut microbiota diversity and community structure in AS patients compared with healthy controls. Notably, the abundance of anti-inflammatory microbiota was increased significantly, accompanied by elevated levels of certain metabolites, such as 6,9,12,15,18,21-tetracosahexaenoic acid and bufadienolide, while levels of urobilin and andrenid acid were significantly reduced. Network analysis further uncovered the significant diferences in microbiota-metabolite interactions between AS patients and healthy controls, indicating gut ecosystem disruption and functional dysfunction in AS.
This study sheds light on the mechanisms of brain-gut axis in AS, suggesting potential microbial and metabolite biomarkers, thus providing valuable insights into AS prediction and treatment.
急性中风（AS）是全球主要的公共卫生问题，具有高发病率、致残率和死亡率。新兴研究表明肠道微生物群及其代谢产物在AS的发病机制、恢复和预后中起关键作用。
在本研究中，我们使用16S rRNA测序和非靶向液相色谱-质谱（LC-MS）代谢组学技术研究了AS患者肠道微生物群组成和代谢组学特征的变化。
结果显示，与健康对照相比，AS患者的肠道微生物群多样性和群落结构有显著变化。值得注意的是，抗炎微生物群的丰度显著增加，同时某些代谢产物水平升高，如6,9,12,15,18,21-二十四碳六烯酸和蟾蜍二烯羟酸内酯，而尿胆素和雄烯二酸水平显著降低。网络分析进一步揭示了AS患者与健康对照之间微生物群-代谢产物相互作用的显著差异，表明AS中肠道生态系统破坏和功能障碍。
本研究揭示了AS中脑-肠轴的机制，提示了潜在的微生物和代谢产物生物标志物，从而为AS的预测和治疗提供了有价值的见解。
