Que Xueyun, He Feiyu, He Qida, Lim Jieying, Li Xiaoshuang, Meng Xianjun, Yang Zongbao
School of Medicine, Xiamen University, Xiamen 361102, Fujian, China.
College of Acupuncture and Moxibustion and Tuina, Fujian University of Traditional Chinese Medicine, Fuzhou 350122, Fujian, China.
Cardiol Res Pract. 2025 Aug 28;2025:7765150. doi: 10.1155/crp/7765150. eCollection 2025.
This study investigates the reparative effect of electroacupuncture on myocardial fibrosis (MF) in mice and explores its impact on intestinal flora and metabolism profile. This examines an investigation into the biological mechanisms underlying electroacupuncture's efficacy in treating MF in mice. Twenty-four male Kunming mice (27-34 g) were randomized into three groups: normal control (NC, = 8), MF model (MF, = 8), and electroacupuncture treatment (EA, = 8). MF and EA groups received daily subcutaneous ISO injections (25 mg/kg) at the nape for 5 days; NC mice received saline. The EA group underwent 14 days of EA at PC6 (Neiguan). Cardiac function, intestinal flora, and metabolites were assessed post-treatment. EA significantly reduced cardiac weight index (CWI), collagen volume fraction (CVF), and serum procollagen III N-terminal propeptide (PIIINP) and improved left ventricular ejection fraction (LVEF) and shortening fraction (LVFS) ( < 0.05). Gut microbiota analysis revealed distinct composition shifts: EA restored abundance and lowered / ratios, resembling NC profiles. Notably, differential bacteria (e.g., , ) correlated with PIIINP, CVF, and cardiac function. Metabolomics identified reduced TMAO, phenylalanine, acetone, and lactic acid in EA vs. MF ( < 0.05). Negative correlations included vs. phenylalanine and vs. acetone. EA ameliorates ISO-induced MF in mice by modulating gut microbiota structure and metabolic profiles, suggesting a microbiota-metabolite axis mediates its therapeutic effects.
本研究探讨电针对小鼠心肌纤维化（MF）的修复作用，并探究其对肠道菌群和代谢谱的影响。本研究还考察了电针治疗小鼠MF疗效的生物学机制。将24只雄性昆明小鼠（27 - 34克）随机分为三组：正常对照组（NC，n = 8）、MF模型组（MF，n = 8）和电针治疗组（EA，n = 8）。MF组和EA组每天在颈部皮下注射异丙肾上腺素（ISO，25毫克/千克），连续注射5天；NC组小鼠注射生理盐水。EA组在双侧内关穴（PC6）进行14天的电针治疗。治疗后评估心功能、肠道菌群和代谢产物。电针显著降低了心脏重量指数（CWI）、胶原容积分数（CVF）以及血清III型前胶原N端前肽（PIIINP），并改善了左心室射血分数（LVEF）和缩短分数（LVFS）（P < 0.05）。肠道微生物群分析显示出明显的组成变化：电针恢复了[具体微生物名称1]丰度并降低了[具体微生物名称2]/[具体微生物名称3]比率，类似于NC组的特征。值得注意的是，差异细菌（如[具体微生物名称4]、[具体微生物名称5]）与PIIINP、CVF和心功能相关。代谢组学分析发现，与MF组相比，EA组的氧化三甲胺（TMAO）、苯丙氨酸、丙酮和乳酸减少（P < 0.05）。负相关包括[具体微生物名称6]与苯丙氨酸以及[具体微生物名称7]与丙酮。电针通过调节肠道微生物群结构和代谢谱改善了ISO诱导的小鼠MF，表明微生物群 - 代谢产物轴介导了其治疗作用。
