MDM4可利用合成RNA从外周血单个核细胞高效生成人诱导多能干细胞。

MDM4 enables efficient human iPS cell generation from PBMCs using synthetic RNAs.

作者信息

Nakagawa Masato, Nogi Mizuho, Doi Hatsuki, Hayashi Ryuhei, Katayama Tomohiko, Ohno Hirohisa, Mochizuki Megumi, Hayashi Karin, Saito Hirohide

机构信息

Center for iPS Cell Research and Application (CiRA), Kyoto University, Kyoto, 606-8507, Japan.

Premium Research Institute for Human Metaverse Medicine (WPI-PRIMe), The University of Osaka, Osaka, 565-0871, Japan.

出版信息

Sci Rep. 2025 Sep 8;15(1):30620. doi: 10.1038/s41598-025-16446-y.

DOI:10.1038/s41598-025-16446-y

PMID:40921827

Abstract

If iPS cells can be established easily and efficiently using freshly collected blood cells, it will enhance regenerative and personalized medicine. While reports of iPS derivation from blood-derived endothelial progenitor cells using RNA have been documented, none have been reported from peripheral blood-derived mononuclear cells (PBMCs). In this study, we established a method to generate iPS cells from PBMCs using synthetic RNAs and found that MDM4, which suppresses p53, improved reprogramming efficiency.

摘要

如果能使用新鲜采集的血细胞轻松高效地建立诱导多能干细胞（iPS细胞），将会促进再生医学和个性化医疗的发展。虽然已有利用RNA从血液来源的内皮祖细胞诱导生成iPS细胞的报道，但尚未有从外周血来源的单核细胞（PBMC）诱导生成iPS细胞的报道。在本研究中，我们建立了一种使用合成RNA从PBMC生成iPS细胞的方法，并发现抑制p53的MDM4可提高重编程效率。