Metzler-Zebeli Barbara U, Sener-Aydemir Arife, Terler Georg, Zebeli Qendrim
Centre for Veterinary Systems Transformation and Sustainability, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
Centre for Animal Nutrition and Welfare, Clinical Department for Farm Animals and Food System Science, University of Veterinary Medicine Vienna, 1210 Vienna, Austria.
JDS Commun. 2025 Jul 16;6(5):700-704. doi: 10.3168/jdsc.2025-0793. eCollection 2025 Sep.
The present study aimed to investigate the effects of feeding different hay qualities with or without concentrate supplementation on the mRNA expression of genes related to hepatic lipid and glucose metabolism and cellular energy status in weaned calves. Holstein Friesian calves (5 per dietary group) were fed 4 solid diets: (1) 100% medium-quality hay (MQH; 9.4 MJ of ME, 149 g CP, 522 g NDF/kg of DM); (2) 100% high-quality hay (HQH; 11.2 MJ of ME, 210 g CP, 455 g NDF/kg of DM); (3) 30% medium-quality hay + 70% starter concentrate (MQHC); and (4) 30% high-quality hay + 70% starter concentrate (HQHC). Calves were slaughtered at 100 ± 4 d of age and an average BW of 128.8 ± 19.8 kg. Liver tissue was collected, snap-frozen in liquid N, and used in the mRNA expression experiment. The trend for lower mRNA expression of AMP-kinase with HQH compared with MQH indicated higher energetic status of hepatocytes with HQH, which probably triggered the increased mRNA expression of enzymes responsible for lipogenesis and cholesterogenesis. The greater hepatic availability of propionate with the hay-concentrate diets may have upregulated the mRNA expression of AMP-kinase by triggering gluconeogenesis. Both HQH and concentrate supplementation downregulated genes related to nutrient catabolism, such as fatty acid oxidation or amino acid breakdown. In conclusion, results demonstrate the importance of the type of solid feed for the energetic state and respective signaling in hepatocytes of weaned calves.
本研究旨在探讨饲喂不同质量干草并补充或不补充精料对断奶犊牛肝脏脂质和葡萄糖代谢相关基因的mRNA表达以及细胞能量状态的影响。荷斯坦弗里生犊牛（每组日粮5头）饲喂4种固体日粮：（1）100%中等质量干草（MQH；每千克干物质含9.4兆焦代谢能、149克粗蛋白、522克中性洗涤纤维）；（2）100%高质量干草（HQH；每千克干物质含11.2兆焦代谢能、210克粗蛋白、455克中性洗涤纤维）；（3）30%中等质量干草+70%开食精料（MQHC）；（4）30%高质量干草+70%开食精料（HQHC）。犊牛在100±4日龄、平均体重128.8±19.8千克时屠宰。采集肝脏组织，在液氮中速冻，用于mRNA表达实验。与MQH相比，HQH组AMP激酶的mRNA表达较低，这表明HQH组肝细胞的能量状态较高，这可能触发了负责脂肪生成和胆固醇生成的酶的mRNA表达增加。干草-精料日粮中丙酸在肝脏中的可用性较高，可能通过触发糖异生作用上调了AMP激酶的mRNA表达。HQH和补充精料均下调了与营养物质分解代谢相关的基因，如脂肪酸氧化或氨基酸分解。总之，结果表明固体饲料类型对断奶犊牛肝细胞的能量状态和相应信号传导具有重要意义。
