Qosimah Dahliatul, Widyaputri Tiara, Ataullah Muhamad Firman, Fu'ada Syifa, Najib Zackya, Farhan Atsir, Putri Fathia Zahra Aulia, Ratuyustiarany Anggieta
Laboratory of Veterinary Microbiology and Immunology, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, East Java 65151, Indonesia.
Veterinary Clinical Pathology and Experimental Animal Laboratory, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, East Java 65151, Indonesia.
Vet World. 2025 Jul;18(7):1955-1963. doi: 10.14202/vetworld.2025.1955-1963. Epub 2025 Jul 17.
is a significant pathogen in freshwater aquaculture, contributing to high morbidity and mortality in common carp (). Conventional reliance on antibiotics raises concerns about resistance and environmental impact. This study aimed to evaluate the effects of short-term fasting (1 or 2 days) on physiological, oxidative stress, and microbial responses in infected with .
Sixty were divided into four groups (n = 15): negative control (uninfected), positive control (infected), T (1-day fasting + infected), and T (2-day fasting + infected). Infections were induced by immersion in an suspension (10 colony-forming units [CFU]/mL). Three days post-infection, blood was collected for glucose and hemoglobin analysis. Malondialdehyde (MDA) levels in head kidney tissue were assessed as a marker of oxidative stress. Gut samples were analyzed for lactic acid bacteria (LAB) through standard plate counts. Statistical comparisons were made using a one-way analysis of variance and Kruskal-Wallis tests (p < 0.05).
The T group (2-day fasting) exhibited significantly better physiological responses than T and the positive control. Blood glucose levels in T (83.5 ± 1.71 mg/dL) were significantly lower than the positive control (127 ± 3.85 mg/dL), but within the normal range. Hemoglobin levels were highest in T (7.8 ± 0.27 g/dL), indicating preserved oxygen-carrying capacity. MDA levels, though not statistically different, were lowest in T (14.42 ± 0.60 mg/L), suggesting reduced oxidative stress. LAB counts were highest in T (1.69 × 10 CFU/g), indicating improved gut microbiota balance.
A 2-day fasting regimen enhanced disease resistance in by modulating glucose metabolism, preserving hematological integrity, reducing oxidative stress, and enriching beneficial gut microbiota. These findings support short-term fasting as a promising non-pharmacological strategy for managing bacterial infections in aquaculture, with the potential to reduce antibiotic dependence.
[病原体名称]是淡水养殖中的一种重要病原体，导致鲤鱼发病率和死亡率居高不下。传统上对抗生素的依赖引发了对耐药性和环境影响的担忧。本研究旨在评估短期禁食（1天或2天）对感染[病原体名称]的鲤鱼生理、氧化应激和微生物反应的影响。
60尾鲤鱼分为四组（n = 15）：阴性对照（未感染）、阳性对照（感染）、T1（1天禁食+感染）和T2（2天禁食+感染）。通过浸泡在[病原体名称]悬浮液（10个菌落形成单位[CFU]/mL）中诱导感染。感染后三天，采集血液进行葡萄糖和血红蛋白分析。评估头肾组织中丙二醛（MDA）水平作为氧化应激的标志物。通过标准平板计数分析肠道样本中的乳酸菌（LAB）。使用单因素方差分析和Kruskal-Wallis检验进行统计比较（p < 0.05）。
T2组（2天禁食）的生理反应明显优于T1组和阳性对照。T2组的血糖水平（83.5±1.71mg/dL）明显低于阳性对照（127±3.85mg/dL），但在正常范围内。血红蛋白水平在T2组最高（7.8±0.27g/dL），表明氧携带能力得以保留。MDA水平虽然无统计学差异，但在T2组最低（14.42±0.60mg/L），表明氧化应激降低。LAB计数在T2组最高（1.69×10[CFU/g]），表明肠道微生物群平衡得到改善。
2天禁食方案通过调节葡萄糖代谢、维持血液学完整性、降低氧化应激和丰富有益肠道微生物群，增强了鲤鱼的抗病能力。这些发现支持短期禁食作为水产养殖中管理细菌感染的一种有前景的非药物策略，有可能减少抗生素依赖。
