Safitri Erma, Purnobasuki Hery, Lestari Tita Damayanti, Utama Suzanita, Rimayanti Rimayanti, Lamid Mirni, Jannah Mutmainah Wardatul, Darodjah Siti, Jang Goo, Takagi Mitsuhiro
Department of Veterinary Science, Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, Indonesia.
Department of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya, Indonesia.
Vet World. 2025 Jul;18(7):1850-1862. doi: 10.14202/vetworld.2025.1850-1862. Epub 2025 Jul 8.
Mycotoxin contamination in poultry feed, particularly with aflatoxin B1 (AFB1) and ochratoxin A (OTA), poses significant threats to broiler health, meat quality, and consumer safety. Toxin binders are commonly used to mitigate these effects; however, their impact on endogenous stem cell activity and overall broiler performance remains underexplored. This study aimed to evaluate the efficacy of a commercial toxin binder in reducing AFB1 and OTA residues in broiler meat, inducing endogenous stem cell production, and improving growth and feed performance indices.
Twenty Cobb broilers were randomly assigned to four groups: Negative control (C-), positive control with mycotoxin-contaminated feed (C+), treatment 1 (T1: 1.1 g/kg binder), and treatment 2 (T2: 1.6 g/kg binder). Broilers were fed for 35 days. AFB1 and OTA levels in pectoral muscles were quantified using high-performance liquid chromatography, while endogenous stem cell markers (CD34+, CD45+, CD105-) in spleen tissue were assessed through flow cytometry. Growth parameters, feed conversion ratio (FCR), and performance index were also evaluated.
AFB1 and OTA residues were significantly reduced in T1 and T2 compared to C+ (p < 0.05), with T2 showing the lowest levels (0.0023 μg/mL and 0.073 μg/mL, respectively). Flow cytometry revealed that T2 significantly induced endogenous stem cells (35.62% ± 2.16) compared to all other groups. The highest average daily growth occurred in T1 (68.78 ± 4.78 g/day), while the best FCR (1.38 ± 0.079) and performance index (386.2 ± 14.34) were also recorded in T1. No mortality occurred in any group.
Administering a toxin binder at 1.6 g/kg effectively reduced AFB1 and OTA residues and significantly activated endogenous stem cells, suggesting a protective and regenerative effect. Meanwhile, a dose of 1.1 g/kg yielded optimal growth performance and feed efficiency. These findings support the dual functional role of toxin binders in enhancing broiler meat safety and physiological resilience.
家禽饲料中的霉菌毒素污染，尤其是黄曲霉毒素B1（AFB1）和赭曲霉毒素A（OTA），对肉鸡健康、肉质和消费者安全构成重大威胁。毒素吸附剂通常用于减轻这些影响；然而，它们对内源性干细胞活性和肉鸡整体性能的影响仍未得到充分研究。本研究旨在评估一种商业毒素吸附剂在降低肉鸡肌肉中AFB1和OTA残留、诱导内源性干细胞生成以及改善生长和饲料性能指标方面的功效。
将20只科宝肉鸡随机分为四组：阴性对照组（C-）、受霉菌毒素污染饲料的阳性对照组（C+）、处理1组（T1：1.1 g/kg吸附剂）和处理2组（T2：1.6 g/kg吸附剂）。肉鸡饲养35天。使用高效液相色谱法定量胸肌中的AFB1和OTA水平，同时通过流式细胞术评估脾脏组织中的内源性干细胞标志物（CD34+、CD45+、CD105-）。还评估了生长参数、饲料转化率（FCR）和性能指标。
与C+组相比，T1组和T2组的AFB1和OTA残留量显著降低（p < 0.05），T2组的残留量最低（分别为0.0023 μg/mL和0.073 μg/mL）。流式细胞术显示，与所有其他组相比，T2组显著诱导了内源性干细胞（35.62% ± 2.16）。T1组的平均日增重最高（68.78 ± 4.78 g/天），同时T1组的最佳FCR（1.38 ± 0.079）和性能指标（386.2 ± 14.34）也被记录到。任何组均未发生死亡。
以1.6 g/kg的剂量施用毒素吸附剂可有效降低AFB1和OTA残留，并显著激活内源性干细胞，表明具有保护和再生作用。同时，1.1 g/kg的剂量产生了最佳的生长性能和饲料效率。这些发现支持了毒素吸附剂在提高肉鸡食品安全和生理恢复力方面的双重功能作用。
