Salary Mohsen, Mohebbi Saleh, Ahmadi Aslan, Bagher Zohreh, Pezeshki-Modaress Mohamad, Aminianfar Hossein, Farzad-Mohajeri Saeed, Samiei Nazanin, Taghizadeh-Hesary Farzad, Ghanbari Hadi
ENT and Head and Neck Research Center and Department, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Skull Base Research Center, The Five Senses Health Institute, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Biomater Biosyst. 2025 Aug 21;19:100120. doi: 10.1016/j.bbiosy.2025.100120. eCollection 2025 Sep.
The airway mucosa plays a crucial role in protection and various physiological functions. Current methods for restoring airway mucosa, such as myocutaneous flaps or split skin grafts, create a stratified squamous layer that lacks the cilia and mucus-secreting glands of the native columnar-lined airway. This study examines the application of various injectable biopolymers as active molecules for a potential approach to regenerating laryngeal epithelial tissue.
The sample includes nine healthy dogs of the same breed. First, the medical engineering team prepared three types of biosynthetic materials (alginate, PGS, and chitosan) in a standard laboratory setting. After the induction of anesthesia in animals, the upper surface of the true vocal cords was bilaterally incised and denuded to create a uniform injury site. Biomaterials were applied to one side (intervention side), while the contralateral side served as the control and received no treatment. The length of the affected area after induction of injury, as observed in the microscopic view, was analyzed in relation to the effects of biomaterials, including epithelial hyperplasia, inflammation, granulation bed formation, angiogenesis, and fibroplasia.
The mean standard deviation (SD) of epithelial hyperplasia scores, inflammatory scores, angiogenesis scores, and fibroplasia scores were not significantly different between the groups. However, the mean (SD) of granulation tissue bed score among the alginate [3.33 (1.15)], PGS [2.33 (0.58)], chitosan [3.33 (0.58)], and control [4.67 (0.58)] groups was significantly different between groups ( = 0.03).
This study demonstrated that a biopolymer has a positive effect on the repair of laryngeal epithelial tissue in an animal model without considering the impact of laryngeal movements.
气道黏膜在保护及多种生理功能中发挥着关键作用。当前恢复气道黏膜的方法，如肌皮瓣或断层皮片移植，会形成一个缺乏天然柱状内衬气道的纤毛和黏液分泌腺的复层鳞状层。本研究探讨了各种可注射生物聚合物作为活性分子在喉上皮组织再生潜在方法中的应用。
样本包括9只同一品种的健康犬。首先，医学工程团队在标准实验室环境中制备了三种生物合成材料（藻酸盐、聚癸二酸甘油酯和壳聚糖）。在动物麻醉诱导后，双侧切开并剥脱真声带的上表面以创建一个均匀的损伤部位。将生物材料应用于一侧（干预侧），而对侧作为对照不进行处理。在显微镜下观察损伤诱导后受影响区域的长度，并分析生物材料的效果，包括上皮增生、炎症、肉芽床形成、血管生成和成纤维细胞增生。
上皮增生评分、炎症评分、血管生成评分和成纤维细胞增生评分的平均标准差（SD）在各组之间无显著差异。然而，藻酸盐组[3.33（1.15）]、聚癸二酸甘油酯组[2.33（0.58）]、壳聚糖组[3.33（0.58）]和对照组[4.67（0.58）]之间的肉芽组织床评分平均值（SD）在组间存在显著差异（ = 0.03）。
本研究表明，在不考虑喉部运动影响的动物模型中，一种生物聚合物对喉上皮组织的修复具有积极作用。
