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结合工程化吡喃糖2-氧化酶和转氨酶用于合成氨基糖的生物催化级联反应。

Biocatalytic cascade combining an engineered pyranose 2-oxidase and transaminases for the synthesis of amino sugars.

作者信息

Chuaboon Litavadee, Visitsatthawong Surawit, Sookbampen Orawan, Suthin Siripakorn, Voss Moritz, Wongnate Thanyaporn, Höhne Matthias, Bornscheuer Uwe T, Chaiyen Pimchai

机构信息

School of Pharmacy, Walailak University Nakhon Si Thammarat 80160 Thailand.

Biomass and Oil Palm Center of Excellence, Walailak University Nakhon Si Thammarat 80160 Thailand.

出版信息

RSC Adv. 2025 Sep 9;15(39):32600-32608. doi: 10.1039/d5ra04556h. eCollection 2025 Sep 5.

DOI:10.1039/d5ra04556h
PMID:40933096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12418926/
Abstract

This work reports cascade reactions using a pyranose 2-oxidase and transaminases for synthesizing the rare amino sugars mannosamine, glucosamine, and galactosamine. Transaminases were engineered to accept unnatural 2-keto-sugars, providing up to 68% galactosamine yield. The mechanisms of C2-amination by these transaminases were also investigated. These transaminase variants have potential applications in high-value amino sugar synthesis.

摘要

这项工作报道了使用吡喃糖2-氧化酶和转氨酶进行级联反应来合成稀有氨基糖甘露糖胺、葡萄糖胺和半乳糖胺。对转氨酶进行了改造，使其能够接受非天然的2-酮糖，半乳糖胺产率高达68%。还研究了这些转氨酶进行C2-氨基化的机制。这些转氨酶变体在高价值氨基糖合成中具有潜在应用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/013d0d443239/d5ra04556h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/827c3731690d/d5ra04556h-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/266a9ff3009d/d5ra04556h-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/85129cb8145d/d5ra04556h-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/013d0d443239/d5ra04556h-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/827c3731690d/d5ra04556h-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/266a9ff3009d/d5ra04556h-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/85129cb8145d/d5ra04556h-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/528f/12418926/013d0d443239/d5ra04556h-f4.jpg

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本文引用的文献

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