Akhtar Muhammad Faheem, Wenqiong Chai, Umar Muhammad, Changfa Wang
Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng, China.
Faculty of Veterinary and Animal Sciences, Department of Animal Reproduction, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, Pakistan.
Front Microbiol. 2025 Aug 26;16:1581430. doi: 10.3389/fmicb.2025.1581430. eCollection 2025.
Ensiling, a microbial-driven process employed for preserving fresh forage in both bio-refineries and animal production, triggers significant biochemical transformations. These changes have spurred the exploration of novel silage additives, with a particular emphasis on the potential of microbial strains that exhibit superior biopreservation capabilities. Lactic acid bacteria (LAB) species have gained widespread recognition for their diverse applications as additives in the fermentation of crops and forage biomasses during ensiling. Nonetheless, recent variations in silage quality might be attributed to a lack of comprehensive information on the gene expression and molecular mechanisms of the microbiota involved in silage production. Contemporary research efforts have been directed toward uncovering nutrient-rich animal feed solutions through enhanced LAB inoculants. This review aims to shed light on the role of LAB inoculants in silage production and the modern biotechnological methods, including metabolomics, proteomics, metagenomics, genomics, transcriptomics, and genetic manipulation. These powerful tools are instrumental in the identification, enhancement, and development of high-performance LAB strains. Additionally, the review outlines emerging trends and prospective developments in LAB advancement for the enhancement of silage, which holds significant promise for breakthroughs in sustainable agriculture and improved animal feed production.
青贮是一种在生物炼制厂和动物生产中用于保存新鲜草料的微生物驱动过程，会引发显著的生化转变。这些变化促使人们探索新型青贮添加剂，尤其关注具有卓越生物保鲜能力的微生物菌株的潜力。乳酸菌（LAB）作为青贮期间作物和草料生物质发酵添加剂的多种应用已得到广泛认可。然而，近期青贮质量的变化可能归因于缺乏关于参与青贮生产的微生物群基因表达和分子机制的全面信息。当代研究工作致力于通过强化乳酸菌接种剂来发现营养丰富的动物饲料解决方案。本综述旨在阐明乳酸菌接种剂在青贮生产中的作用以及现代生物技术方法，包括代谢组学、蛋白质组学、宏基因组学、基因组学、转录组学和基因操作。这些强大的工具有助于鉴定、增强和开发高性能乳酸菌菌株。此外，该综述概述了乳酸菌在青贮改良方面的新趋势和未来发展，这对于可持续农业的突破和动物饲料生产的改善具有重大前景。
