Li Guangjing, Qi Jie, Xu Wenying, Chen Linlin, Nyande Ashadu, Xie Zhouli, Gu Jiangjiang, Li Zhaohu, Wu Honghong
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, The Center of Crop Nanobiotechnology, College of Plant Science & Technology Huazhong Agricultural University Wuhan 430070 China.
Hubei Hongshan Laboratory Wuhan 430070 China.
Glob Chall. 2025 Jul 20;9(9):e00186. doi: 10.1002/gch2.202500186. eCollection 2025 Sep.
Lateral root (LR) formation is important for plant growth. ROS (reactive oxygen species)play an important role in LR formation. While how nanomaterials affect ROS distribution to promote LR formation and the role of ROS in primordia in LR formation are rarely known. Cerium oxide nanoparticles (nanoceria), as a potent ROS scavenger, are widely used in plants. This study investigates the effects of poly (acrylic acid) nanoceria (PNC, 6.5 nm, -36 mV), aminated nanoceria (ANC, 6.9 nm, 30 mV), and bulk nanoceria (BNC, 84.9 nm, -5.5 mV) on LR formation in . Only PNC increased LR numbers by 73.5%, reducing root HO levels by up to 90.44% and altering O₂ distribution in LR primordia (LRP). Furthermore, DPI (diphenyleneiodonium, O₂ inhibitor) decreased LR numbers by 18.9%, while PNC treatment reversed this inhibition (12.25 ± 0.53 vs 8.38 ± 0.52). Transcriptome analysis shows PNC regulated ROS metabolism via genes like peroxiredoxins and peroxidases, promoting LR formation. Interestingly, PNC does not affect auxin distribution (confirmed by DR5pro::GFP lines) or alleviate NPA-induced (N-1-naphthylphthalamic acid, an auxin transport inhibitor) LR inhibition. These findings suggest that PNC enhances LR formation through ROS modulation rather than auxin signaling.
侧根（LR）的形成对植物生长很重要。活性氧（ROS）在侧根形成中起重要作用。然而，纳米材料如何影响ROS分布以促进侧根形成以及ROS在侧根形成原基中的作用却鲜为人知。氧化铈纳米颗粒（纳米氧化铈）作为一种有效的ROS清除剂，被广泛应用于植物中。本研究调查了聚（丙烯酸）纳米氧化铈（PNC，6.5纳米，-36毫伏）、胺化纳米氧化铈（ANC，6.9纳米，30毫伏）和块状纳米氧化铈（BNC，84.9纳米，-5.5毫伏）对拟南芥侧根形成的影响。只有PNC使侧根数量增加了73.5%，使根部HO水平降低了90.44%，并改变了侧根原基（LRP）中的O₂分布。此外，二苯基碘鎓（DPI，O₂抑制剂）使侧根数量减少了18.9%，而PNC处理逆转了这种抑制作用（12.25±0.53对8.38±0.52）。转录组分析表明，PNC通过过氧化物酶和过氧化物酶等基因调节ROS代谢，促进侧根形成。有趣的是，PNC不影响生长素分布（由DR5pro::GFP系证实），也不能缓解NPA诱导的（N-1-萘基邻苯二甲酸，一种生长素运输抑制剂）侧根抑制。这些发现表明，PNC通过调节ROS而不是生长素信号来增强侧根形成。
