National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, The Center of Crop Nanobiotechnology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; and Hubei Hongshan Laboratory, Wuhan 430070, China.
National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, The Center of Crop Nanobiotechnology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; and Hubei Hongshan Laboratory, Wuhan 430070, China; and Shenzhen Institute of Nutrition and Health, Huazhong Agricultural University, Shenzhen 511464, China; and Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 511464, China.
Funct Plant Biol. 2024 Oct;51. doi: 10.1071/FP24196.
Roots play an important role in plant growth, including providing essential mechanical support, water uptake, and nutrient absorption. Nanomaterials play a positive role in improving plant root development, but there is limited knowledge of how nanomaterials affect lateral root (LR) formation. Poly (acrylic) acid coated nanoceria (cerium oxide nanoparticles, PNC) are commonly used to improve plant stress tolerance due to their ability to scavenge reactive oxygen species (ROS). However, its impact on LR formation remains unclear. In this study, we investigated the effects of PNC on LR formation in Arabidopsis thaliana by monitoring ROS levels and Ca2+ distribution in roots. Our results demonstrate that PNC significantly promote LR formation, increasing LR numbers by 26.2%. Compared to controls, PNC-treated Arabidopsis seedlings exhibited reduced H2 O2 levels by 18.9% in primary roots (PRs) and 40.6% in LRs, as well as decreased O 2 · - levels by 47.7% in PRs and 88.5% in LRs. When compared with control plants, Ca2+ levels were reduced by 35.7% in PRs and 22.7% in LRs of PNC-treated plants. Overall, these results indicate that PNC could enhance LR development by modulating ROS and Ca2+ levels in roots.
根在植物生长中起着重要作用，包括提供必要的机械支撑、水分吸收和养分吸收。纳米材料在改善植物根系发育方面发挥着积极作用，但对于纳米材料如何影响侧根（LR）形成的知识有限。由于聚（丙烯酸）酸包覆的纳米氧化铈（氧化铈纳米颗粒，PNC）能够清除活性氧（ROS），因此通常被用于提高植物的抗胁迫能力。然而，其对 LR 形成的影响尚不清楚。在这项研究中，我们通过监测根中的 ROS 水平和 Ca2+分布，研究了 PNC 对拟南芥 LR 形成的影响。我们的结果表明，PNC 可显著促进 LR 形成，使 LR 数量增加 26.2%。与对照相比，PNC 处理的拟南芥幼苗中，PR 和 LR 中的 H2 O2 水平分别降低了 18.9%和 40.6%，PR 和 LR 中的 O 2 · -水平分别降低了 47.7%和 88.5%。与对照植物相比，PNC 处理的植物 PR 和 LR 中的 Ca2+水平分别降低了 35.7%和 22.7%。总的来说，这些结果表明 PNC 可以通过调节根中的 ROS 和 Ca2+水平来增强 LR 的发育。
