Centrifuge-Free Bone Marrow Aspirate Harvest for Orthopaedic Applications.

Autologous bone marrow aspirate (BMA) is increasingly being used in clinical practice to augment the repair and healing of bone, cartilage, and tendon by delivering mesenchymal stem cells (MSCs) and growth factors to the site of injury. Traditional BMA harvest and preparation rely on centrifugation to generate a BMA concentrate with a higher concentration of MSCs than the original aspirate. However, this traditional technique may be inefficient and time-consuming, and it could sacrifice sterility. In this technique, we describe a centrifuge-free BMA harvest system (Marrow Cellution; Cervos Medical) that avoids peripheral blood dilution during aspiration, thereby potentially achieving a higher yield of MSCs and growth factors within the BMA without the need for centrifugation.