Duan Ying, Bo Kailiang, Shu Qin, Zhang Meng, Shi Yuzi, Weng Yiqun, Wang Changlin
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, College of Horticulture, China Agricultural University, Beijing, 100193 China.
Mol Breed. 2025 Sep 9;45(9):74. doi: 10.1007/s11032-025-01592-y. eCollection 2025 Sep.
Zucchini ( subsp. ) stands as an economically vital crop in China. In zucchini breeding, plant architectural patterns and fruit morphological characteristics serve as pivotal traits. In this study, we employed quantitative trait locus (QTL) analysis using recombinant inbred lines (RILs) derived from two distinct inbred lines, JinGL (subsp. ) and HM-S2 (subsp. ), in conjunction with a high-density genetic map. Our investigation focused on ten QTLs associated with six horticulturally significant traits, including hypocotyl length (HL), plant height (PH), and four fruit-related traits: fruit length (FL), fruit diameter (FD), fruit shape index (FSI), and fruit weight (FW). The QTLs governing HL and PH were mapped to Chr03/LG10 and named and , respectively. The candidate gene / for was successfully identified. Additionally, three novel QTLs related to fruit size and shape were discovered. Among them, , demarcated by Marker238258 and Marker240069 on Chromosome 08/Linkage group 17 (Chr08/LG17), is a new major QTL regulating the fruit shape of zucchini. Through genomic insertion-deletion (InDel) and qRT-PCR analyses, we predicted genes within the candidate interval, uncovering and as potential candidate genes. We developed molecular markers tightly linked to and and validated them in 171 and 224 germplasms, achieving accuracy rates of 96% and 100%, respectively. This study deepens our understanding of the genetic basis of key traits and provides valuable references for molecular breeding in .
The online version contains supplementary material available at 10.1007/s11032-025-01592-y.
西葫芦（亚种）是中国一种具有重要经济价值的作物。在西葫芦育种中，植株形态模式和果实形态特征是关键性状。在本研究中，我们利用从两个不同自交系金GL（亚种）和HM - S2（亚种）衍生而来的重组自交系（RILs），结合高密度遗传图谱进行数量性状位点（QTL）分析。我们的研究重点是与六个园艺学重要性状相关的10个QTL，包括下胚轴长度（HL）、株高（PH）以及四个果实相关性状：果实长度（FL）、果实直径（FD）、果实形状指数（FSI）和果实重量（FW）。控制HL和PH的QTL分别定位到Chr03/LG10上，命名为 和 。成功鉴定了 的候选基因 / 。此外，还发现了三个与果实大小和形状相关的新QTL。其中，由染色体08/连锁群17（Chr08/LG17）上的标记238258和标记240069界定的 是调控西葫芦果实形状的一个新的主效QTL。通过基因组插入缺失（InDel）和qRT - PCR分析，我们预测了 候选区间内的基因，发现 和 是潜在的候选基因。我们开发了与 和 紧密连锁的分子标记，并在171份和224份种质资源中进行了验证，准确率分别达到96%和100%。本研究加深了我们对关键性状遗传基础的理解，并为西葫芦的分子育种提供了有价值的参考。
在线版本包含可在10.1007/s11032 - 025 - 01592 - y获取的补充材料。
