Xu Nuo, Fu Haijing, Liu Yujia, Kilaru Aruna, Behera Jyoti R, Wang Ling
College of Landscape Architecture, Northeast Forestry University, Harbin 150040, China.
Department of Biological Sciences, East Tennessee State University, Johnson City, TN 37614, USA.
Plants (Basel). 2025 Sep 2;14(17):2733. doi: 10.3390/plants14172733.
is an ornamental plant and so its wild genetic resources need to be protected. However, traditional inefficient propagation limits its landscape applications. In this study, we assessed the effects of phytohormones on growth of at various culture stages using roots and hypocotyls as explants and established an efficient micropropagation system. The highest callus induction of hypocotyl (75.0%) was obtained using Murashige and Skoog medium containing 6-benzylaminopurine (6-BA), 0.5 mg L + 2,4-dichlorophenoxyacetic acid (2,4-D), 1.0 mg L + 1-naphthylacetic acid (NAA), and 0.4 mg L. Similarly, the highest callus induction (73.3%) of roots was achieved with 6-BA 0.5 mg L + 2,4-D 0.5 mg L + NAA 0.4 mg L. The calli induced from hypocotyl and root tissues achieved 39.7% and 49.5% adventitious shoot induction on a medium containing indole-3-butyric acid (IBA) 0.5 mg L + 6-BA 1.5 mg L + NAA 1.0 mg L and 6-BA 2.0 mg L + NAA 0.4 mg L + kinetin (KT) 1.0 mg L, respectively. The rooting of adventitious shoots reached 93.3% in the medium supplemented with NAA 0.2 mg L. The survival of regenerated plants reached 90.0% after being transplanted into soil. This study provides an efficient and reliable propagation method for for landscape applications and the preservation of wild genetic material.
是一种观赏植物，因此其野生遗传资源需要得到保护。然而，传统的低效繁殖方式限制了其在园林中的应用。在本研究中，我们以根和下胚轴为外植体，评估了植物激素在不同培养阶段对其生长的影响，并建立了高效的微繁殖体系。使用含有6-苄基腺嘌呤（6-BA）0.5 mg/L、2,4-二氯苯氧乙酸（2,4-D）1.0 mg/L、1-萘乙酸（NAA）0.4 mg/L的Murashige和Skoog培养基，下胚轴的愈伤组织诱导率最高（75.0%）。同样，使用6-BA 0.5 mg/L + 2,4-D 0.5 mg/L + NAA 0.4 mg/L时，根的愈伤组织诱导率最高（73.3%）。在下胚轴和根组织诱导的愈伤组织上，分别在含有吲哚-3-丁酸（IBA）0.5 mg/L + 6-BA 1.5 mg/L + NAA 1.0 mg/L和6-BA 2.0 mg/L + NAA 0.4 mg/L + 激动素（KT）1.0 mg/L的培养基上，不定芽诱导率分别达到39.7%和49.5%。在添加NAA 0.2 mg/L的培养基中，不定芽的生根率达到93.3%。再生植株移栽到土壤后，成活率达到90.0%。本研究为其园林应用和野生遗传材料的保存提供了一种高效可靠的繁殖方法。
