The College of Landscape Architecture, Northeast Forestry University, Harbin, 150040, China.
Department of Biological Sciences, East Tennessee State University, Johnson City, TN, 37614, USA.
Sci Rep. 2018 Nov 20;8(1):17100. doi: 10.1038/s41598-018-35281-y.
Iris sanguinea is a perennial flowering plant that is typically cultivated through seeds or bulbs. However, due to limitations in conventional propagation, an alternate regeneration system using seeds was developed. The protocol included optimization of sterilization, stratification and scarification methods as iris seeds exhibit physiological dormancy. In addition to chlorine-based disinfection, alkaline or heat treatment was used to break seed dormancy and reduce contamination. When seeds were soaked in water at 80 °C overnight, and sterilized with 75% EtOH for 30 s and 4% NaOCl solution for 20 minutes, contamination was reduced to 10% and a 73.3% germination was achieved. The germinated seedlings with 2-3 leaves and radicle were used as explants to induce adventitious buds. The optimal MS medium with 0.5 mg L 6-benzylaminopurine, 0.2 mg L NAA, and 1.0 mg L kinetin resulted in 93.3% shoot induction and a proliferation coefficient of 5.30. Medium with 0.5 mg L NAA achieved 96.4% rooting of the adventitious shoots. The survival rate was more than 90% after 30 days growth in the cultivated matrix. In conclusion, a successful regeneration system for propagation of I. sanguinea was developed using seeds, which could be utilized for large-scale propagation of irises of ecological and horticultural importance.
血根鸢尾是一种多年生开花植物，通常通过种子或鳞茎进行栽培。然而，由于常规繁殖的限制，开发了一种使用种子的替代再生系统。该方案包括优化消毒、层积和刻伤方法，因为鸢尾种子表现出生理休眠。除了基于氯的消毒外，还使用碱性或热处理来打破种子休眠并减少污染。当种子在 80°C 的水中浸泡过夜，并用 75%乙醇消毒 30 秒，4%次氯酸钠溶液消毒 20 分钟时，污染减少到 10%，发芽率达到 73.3%。用 0.5mg/L 6-苄基氨基嘌呤、0.2mg/L NAA 和 1.0mg/L 激动素的最佳 MS 培养基诱导不定芽，诱导率为 93.3%，增殖系数为 5.30。不定芽的生根率在含有 0.5mg/L NAA 的培养基中达到 96.4%。在培养基质中生长 30 天后，成活率超过 90%。总之，开发了一种成功的血根鸢尾种子繁殖再生系统，可用于生态和园艺重要的鸢尾的大规模繁殖。
