Chen Fangjun, Wang Wei, Liu Wenli, Song Jiancheng, Chen Shihua, An Yibo, Yin Haibo, Guo Shanli
College of Life Sciences, Yantai University, Yantai 264005, China.
Shandong Laboratory of Advanced Agriculture Sciences at Weifang, Peking University Institute of Advanced Agricultural Sciences, Weifang 261325, China.
Plants (Basel). 2025 Sep 4;14(17):2770. doi: 10.3390/plants14172770.
Heat shock protein 90 (HSP90) is an evolutionarily conserved molecular chaperone. Numerous studies have shown that it is widely involved in protein folding, assembly, stabilization, activation, and degradation in response to various biotic and abiotic stresses in many normal cellular processes and under stress conditions. We identified 11 members of the gene family in the quinoa () genome by bioinformatics analysis. Phylogenetic tree analysis showed that quinoa was more closely related to dicotyledonous plants than to monocotyledonous plants. Quinoa is susceptible to high-temperature stress during its growth and development. We analyzed the cis-acting elements of its promoter, and found that nearly 1/3 of the cis-acting elements were stress-responsive, and 2/3 of them had heat-responsive elements. The results of qRT-PCR showed that heat shock at 40 °C could induce a high expression of and . Subcellular localization indicates that they are all membrane proteins. At the same time, both and overexpression lines improved the tolerance of under high temperature, indicating that both of them had a positive regulatory effect under heat stress. Results of this study could provide useful information for further study on the biological role of and , and provide theoretical basis for quinoa resistance breeding.
热休克蛋白90（HSP90）是一种进化上保守的分子伴侣。大量研究表明，在许多正常细胞过程和应激条件下，它广泛参与蛋白质折叠、组装、稳定、激活和降解，以应对各种生物和非生物胁迫。通过生物信息学分析，我们在藜麦（）基因组中鉴定出该基因家族的11个成员。系统发育树分析表明，藜麦与双子叶植物的关系比与单子叶植物的关系更密切。藜麦在生长发育过程中易受高温胁迫。我们分析了其启动子的顺式作用元件，发现近1/3的顺式作用元件是胁迫响应元件，其中2/3具有热响应元件。qRT-PCR结果表明，40℃热激可诱导和的高表达。亚细胞定位表明它们都是膜蛋白。同时，和过表达系均提高了藜麦在高温下的耐受性，表明它们在热胁迫下均具有正向调节作用。本研究结果可为进一步研究和的生物学作用提供有用信息，为藜麦抗性育种提供理论依据。
