Heo Tae Hyeon, Park Hyeon, Cho Jungeun, Lee Da Hyeon, Lee Ju Kyong
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, Republic of Korea.
Interdisciplinary Program in Smart Agriculture, Kangwon National University, Chuncheon 24341, Republic of Korea.
Plants (Basel). 2025 Sep 6;14(17):2799. doi: 10.3390/plants14172799.
To identify SSR markers associated with both quantitative and qualitative traits in , we analyzed a total of 68 individuals from an F population derived from a cross between WPC06-339 (weedy var. ) and WPF17-049 (weedy var. ) using 40 SSR primer sets. The genetic diversity of these markers ranged from 0.464 to 0.676, with a mean value of 0.607. Correlation analysis of 13 morphological traits (4 qualitative, 9 quantitative) revealed significant positive correlations among three leaf-related traits and two inflorescence-related traits. Association analysis involving 40 SSR markers and the 13 morphological traits identified 39 significant marker-trait associations, comprising 18 SSR markers associated with 11 morphological traits. Among these SSR markers, 12 were associated with two to five quantitative or qualitative traits. Additionally, 10 SSR markers were significantly associated with three qualitative traits, while 15 SSR markers were associated with eight quantitative traits. Notably, GBPFM179, KNUPF59, and KNUPF167 were significantly associated with multiple quantitative or qualitative traits. GBPFM179 and KNUPF182 exhibited the highest R values, of 0.38, for stem color and days to maturity, respectively. These SSR markers demonstrate the potential for use in marker-assisted selection in breeding programs aimed at enhancing leaf or seed productivity through the selection of both quantitative and qualitative traits.
为了鉴定与[物种名称]中数量性状和质量性状均相关的简单序列重复（SSR）标记，我们使用40个SSR引物组分析了来自WPC06 - 339（杂草变种[具体变种名1]）和WPF17 - 049（杂草变种[具体变种名2]）杂交产生的F群体的总共68个个体。这些标记的遗传多样性范围为0.464至0.676，平均值为0.607。对13个形态性状（4个质量性状，9个数量性状）的相关性分析显示，三个与叶相关的性状和两个与花序相关的性状之间存在显著正相关。涉及40个SSR标记和13个形态性状的关联分析确定了39个显著的标记 - 性状关联，包括与11个形态性状相关的18个SSR标记。在这些SSR标记中，12个与两到五个数量或质量性状相关。此外，10个SSR标记与三个质量性状显著相关，而15个SSR标记与八个数量性状相关。值得注意的是，GBPFM179、KNUPF59和KNUPF167与多个数量或质量性状显著相关。GBPFM179和KNUPF182分别在茎颜色和成熟天数方面表现出最高的R值，均为0.38。这些SSR标记表明，在旨在通过选择数量和质量性状来提高叶或种子生产力的[物种名称]育种计划中，它们具有用于标记辅助选择的潜力。
