Partial Replacement of Peat: Effects on Substrate Physico-Hydrological Properties and Sage Growth.

The transformation of organic by-products derived from waste into value-added resources represents a promising strategy to advance circular economy principles and bolster environmental and agricultural sustainability, especially in soilless cultivation. This study evaluates the viability of three organic by-products-wood fiber (WF), coffee silverskin (CS), and brewer's spent grains (BSGs)-as partial peat replacements in horticultural substrates. Ten growing media formulations were assessed, incorporating increased doses (0-40% / as peat replacement-PR) of each alternative by-product. The effects on physical and hydraulic substrate properties, along with plant growth traits, were examined using two ornamental genotypes, 'Victoria' and 'Amistad'. To synthesize the multivariate growth data into a single, biologically meaningful metric, based on the first principal component, a Growth Index (GI), a PC1-derived index, was calculated, providing a powerful, unified metric to rank substrate efficacy. WF-based substrates exhibited increased porosity and diminished water retention, whereas media enriched with CS and BSG enhanced moisture availability, particularly at 20-40 PR. The bulk density was highest at PR40 for both WF and BSG treatments, and at PR20 in CS-based substrates. Electrical conductivity increased in CS and BSG treatments with rising PR levels. The results on the vegetative growth of ornamental sages have highlighted that differential PR rates are required depending on the specific organic by-product and plant genotype. In 'Victoria', GI indicates that a 20% replacement of peat with BSG provided the optimal conditions for holistic plant development; the lowest GI for WF substrates across nearly all peat replacement levels indicated that it was the most detrimental alternative for this cultivar. In 'Amistad', the analysis of the GI scores revealed that the CS20 and BSG20 of peat replacement yielded the highest overall growth, with GI scores significantly greater than those of the peat control. CS10 and BSG40 also showed high GI scores in 'Amistad'. WF10 had GI scores similar to those of the peat control. In general, the GI-based approach confirms that moderate inclusion of brewer's spent grain (BSG20) is a highly effective peat replacement for both genotypes. At the same time, coffee silverskin (CS) is particularly effective for the 'Amistad' genotype. This analysis underscores that optimal substrate formulation is not only dependent on the amendment type and rate but also critically on the plant genotype.