Xiong Yun-Shan, Cui Ling-Ling, Hu Gui-Lei, Jiang Yu-Tai, Lv Yong-Po, Zhang Pei, Zheng Jin-Song, Zhang Bai-Zhong, Liu Run-Qiang
Hebi Institute of Engineering and Technology, Henan Polytechnic Univ, Hebi, China.
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang, China.
Neotrop Entomol. 2025 Jan 14;54(1):23. doi: 10.1007/s13744-024-01239-1.
Sitobion miscanthi is a wheat aphid species that can damage seriously agricultural production. The effective management of wheat aphids has depended on chemical insecticides. However, their wide application led to severe resistance of wheat aphids to some insecticides, and cytochrome P450 as a detoxifying enzyme plays a crucial role in the insecticide resistance. In this study, CYP6CY1, a new P450 gene, was isolated and overexpressed in a S.miscanthi resistant strain to imidacloprid. The increased sensitivity to imidacloprid after silencing of CYP6CY1 indicated that its involvement in imidacloprid resistance. Subsequently, action of miR-3047-3p within the 3' UTR of the CYP6CY1 was confirmed at the posttranscriptional regulatory level, and was shown to be involved in the resistance to imidacloprid. These results provide opportunities for understanding the roles of P450 in insecticide resistance of pests.
麦长管蚜是一种会严重损害农业生产的小麦蚜虫物种。对小麦蚜虫的有效治理一直依赖于化学杀虫剂。然而，它们的广泛应用导致小麦蚜虫对某些杀虫剂产生了严重抗性，而细胞色素P450作为一种解毒酶在抗药性中起着关键作用。在本研究中，一个新的P450基因CYP6CY1在对吡虫啉具有抗性的麦长管蚜品系中被分离并过表达。CYP6CY1沉默后对吡虫啉的敏感性增加，表明其参与了对吡虫啉的抗性。随后，在转录后调控水平证实了miR-3047-3p在CYP6CY1的3'UTR内的作用，并表明其参与了对吡虫啉的抗性。这些结果为理解P450在害虫抗药性中的作用提供了契机。
