Niemi S M, Fox J G, Brown L R, Langer R
Lab Anim Sci. 1985 Dec;35(6):609-12.
Ethylene-vinyl acetate copolymer (EVAc) was evaluated as an antigen delivery device in laboratory rabbits. Bovine serum albumin (BSA) was incorporated with EVAc in a pellet, which was implanted subcutaneously. Serum antibody titers to BSA in four implanted rabbits were equal to titers in four rabbits injected twice with BSA in complete Freund's adjuvant. Three of four rabbits implanted with EVAc displayed no inflammation or systemic illness in response to the pellet. The fourth rabbit repeatedly developed a small abscess at the implantation site, but the lesions were less severe than complete Freund's adjuvant injection sites. The EVAc pellet is recommended as a non-inflammatory alternative method to Freund's adjuvants for producing serum antibody in rabbits.
乙烯-醋酸乙烯酯共聚物（EVAc）在实验兔中被评估为一种抗原递送装置。牛血清白蛋白（BSA）与EVAc混合制成小丸，皮下植入。四只植入小丸的兔子中针对BSA的血清抗体滴度与四只在完全弗氏佐剂中注射两次BSA的兔子的滴度相当。四只植入EVAc的兔子中有三只对小丸未表现出炎症或全身疾病反应。第四只兔子在植入部位反复出现小脓肿，但病变程度比完全弗氏佐剂注射部位轻。推荐使用EVAc小丸作为一种无炎症的替代方法，以替代弗氏佐剂在兔中产生血清抗体。
