Yokoyama Sayaka, Kikuchi Amane, Takahashi Hideaki, Ushimaru Hinako, Yamaguchi Hibiki, Yamada Chikako, Fujiki Kotoyo, Kozai Hana, Ota Suzuno, Fujii Tadashi, Hirooka Yoshiki, Tochio Takumi, Tanaka Mamoru
Graduate School of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto, Kasugai 487-8501, Japan.
Department of Food and Nutritional Environment, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya 463-8521, Japan.
Nutrients. 2025 Aug 31;17(17):2844. doi: 10.3390/nu17172844.
: cladodes are rich in dietary fiber, polyphenols, and minerals, which are known to exert antioxidant and immunomodulatory effects. However, the mechanisms and active constituents have not been fully elucidated. In this study, we investigated the effects of continuous . consumption on lipid metabolism, immune function, and the gut microbiota in mice. The feed was made using freeze-dried and powdered cladodes of . Male C57BL/6J mice were assigned to four groups: control diet (C), control diet plus 10% (CN), high-fat diet (FC), and high-fat diet plus 10% (FN). Cactus supplementation reduced the body and liver weights that were elevated by the high-fat diet. Serum total cholesterol and free fatty acids were increased in the FC group compared with the C group, while cactus intake lowered these levels and enhanced fecal cholesterol excretion. Cactus consumption also elevated fecal total IgA and mucin contents. IL-4 expression in Peyer's patches was significantly increased in the FN group compared with the FC group. Gut microbiota analysis showed significant differences in β-diversity, along with increased α-diversity and higher abundance of following cactus intake. These findings suggest that intake increases gut microbiota diversity, which enhances intestinal barrier function and thereby contributes to improved lipid metabolism and immune regulation.
叶状枝富含膳食纤维、多酚和矿物质，已知这些成分具有抗氧化和免疫调节作用。然而，其作用机制和活性成分尚未完全阐明。在本研究中，我们调查了连续食用对小鼠脂质代谢、免疫功能和肠道微生物群的影响。饲料是用冻干的叶状枝粉末制成的。雄性C57BL/6J小鼠被分为四组：对照饮食（C）、对照饮食加10%（CN）、高脂饮食（FC）和高脂饮食加10%（FN）。补充仙人掌可降低高脂饮食导致升高的体重和肝脏重量。与C组相比，FC组血清总胆固醇和游离脂肪酸增加，而摄入仙人掌可降低这些水平并增加粪便胆固醇排泄。食用仙人掌还可提高粪便中总IgA和粘蛋白含量。与FC组相比，FN组派尔集合淋巴结中IL-4表达显著增加。肠道微生物群分析显示，摄入仙人掌后，β多样性存在显著差异，同时α多样性增加，且[具体微生物名称]丰度更高。这些发现表明，摄入仙人掌可增加肠道微生物群多样性，从而增强肠道屏障功能，进而有助于改善脂质代谢和免疫调节。
