Casajús Victoria, Bárcena Alejandra, Martínez Gustavo, Costa Lorenza
Instituto de Fisiología Vegetal, Universidad Nacional de La Plata- Consejo Nacional de Investigaciones Científicas y Tecnológicas, Buenos Aires, Argentina.
Mol Biol Rep. 2025 Sep 13;52(1):903. doi: 10.1007/s11033-025-11030-z.
Kale is a leafy vegetable rich in phytochemicals that promote health. After harvest, leaf senescence is induced, and kale turns yellow due to the degradation of chlorophyll. Previous studies have shown that low-intensity red light pulses delay postharvest senescence in kale. This effect may be mediated by phytochromes activated by red light. The effect of low-intensity red-light LED treatment on the expression of genes involved in chlorophyll degradation (NYC1, NOL, SGR, PPH, and PAO) and genes encoding senescence-associated transcription factors related to the phytochrome signaling cascade (PIF4, ORE1, and WRKY6) was examined in this work.
Kale leaves were treated daily with red-light-LED (22 ± 3 µmol m s) for 1 h and then were stored in the dark together with untreated leaves. Samples were taken during storage to perform biochemical and gene analysis. A close correlation was found between chlorophyll degradation and the gene expression of NYC1, SGR, and PAO in control leaves. Red light treatment caused a strong inhibition of these gene expressions, which explains the retention of chlorophylls. PIF4 and WRKY6 were not upregulated at the expression level in the dark, while red light induced that expression only for a short time. ORE1 expression increased in darkness and was inhibited by red treatment.
Red light treatment preserved chlorophyll by the strong inhibition of NYC1, SGR, and PAO expression in irradiated leaves. The expression of ORE1 increased in darkness and was inhibited by red treatment, as was expected in the red light-activated phytochrome signaling cascade.
羽衣甘蓝是一种富含促进健康的植物化学物质的叶菜类蔬菜。收获后，叶片会诱导衰老，由于叶绿素降解，羽衣甘蓝会变黄。先前的研究表明，低强度红光脉冲可延缓羽衣甘蓝采后的衰老。这种效应可能由红光激活的光敏色素介导。本研究检测了低强度红光发光二极管（LED）处理对参与叶绿素降解的基因（NYC1、NOL、SGR、PPH和PAO）以及与光敏色素信号级联相关的衰老相关转录因子编码基因（PIF4、ORE1和WRKY6）表达的影响。
每天用红光LED（22±3 μmol m² s⁻²）处理羽衣甘蓝叶片1小时，然后与未处理的叶片一起在黑暗中储存。在储存期间取样进行生化和基因分析。在对照叶片中发现叶绿素降解与NYC1、SGR和PAO的基因表达之间存在密切相关性。红光处理强烈抑制了这些基因的表达，这解释了叶绿素的保留。PIF4和WRKY6在黑暗中表达水平未上调，而红光仅在短时间内诱导其表达。ORE1的表达在黑暗中增加，并受到红光处理的抑制。
红光处理通过强烈抑制照射叶片中NYC1、SGR和PAO的表达来保留叶绿素。正如在红光激活的光敏色素信号级联中所预期的那样，ORE1的表达在黑暗中增加，并受到红光处理的抑制。
