Prado Paula F V, Mendes Caio V C, Pires Bárbara A, Fiorin Gabriel L, Mieczkowski Piotr, Pereira Gonçalo A G, Teixeira Paulo J P L, Thomazella Daniela P T
Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo 13083970, Brazil.
Department of Biology, "Luiz de Queiroz" College of Agriculture (ESALQ), University of São Paulo (USP), Piracicaba, São Paulo 13418900, Brazil.
iScience. 2025 Jul 23;28(8):113180. doi: 10.1016/j.isci.2025.113180. eCollection 2025 Aug 15.
Strobilurin fungicides inhibit mitochondrial respiration, leading to ATP depletion and oxidative stress. Although widely used in agriculture, these chemicals are ineffective against the cacao pathogen . Here, we show that tolerates high concentrations of the commercial strobilurin azoxystrobin . Transcriptomic analysis revealed that short-term exposure triggers upregulation of genes related to catabolic pathways, including the glyoxylate cycle and fatty acid degradation, alongside repression of genes involved in anabolic processes, such as cell division and ribosome biogenesis. Simultaneously, genes associated with cellular detoxification and oxidative stress responses were strongly induced. These alterations suggest that remodels its metabolism to counteract fungicide toxicity. Remarkably, long-term exposure to azoxystrobin led to the emergence of a resistant mutant harboring mutations in two putative growth and transcriptional regulators. This work provides new insights into the molecular basis of strobilurin resistance and informs strategies for more effective fungicide deployment in agriculture.
甲氧基丙烯酸酯类杀菌剂抑制线粒体呼吸，导致ATP耗竭和氧化应激。尽管这些化学物质在农业中广泛使用，但它们对可可病原体无效。在这里，我们表明[具体名称未给出]能够耐受高浓度的商业甲氧基丙烯酸酯类杀菌剂嘧菌酯。转录组分析表明，短期暴露会触发与分解代谢途径相关的基因上调，包括乙醛酸循环和脂肪酸降解，同时抑制参与合成代谢过程的基因，如细胞分裂和核糖体生物发生。同时，与细胞解毒和氧化应激反应相关的基因被强烈诱导。这些变化表明[具体名称未给出]重塑其代谢以对抗杀菌剂毒性。值得注意的是，长期暴露于嘧菌酯导致出现一个抗性突变体，该突变体在两个假定的生长和转录调节因子中发生了突变。这项工作为甲氧基丙烯酸酯类抗性的分子基础提供了新的见解，并为农业中更有效使用杀菌剂的策略提供了信息。
