Lv Fengxia, Li Pan, Wang Bin, Yuan Xiaobing, Wang Xu, Dong Shishan
College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou, Henan, China.
College of Veterinary Medicine, Hebei Agricultural University, Baoding, China.
Front Vet Sci. 2025 Aug 29;12:1616237. doi: 10.3389/fvets.2025.1616237. eCollection 2025.
(DXY), a widely used traditional Chinese medicinal component, can be used as an alternative for the reduction of veterinary antibiotic use. But few studies have explored its anti-inflammatory effects and toxicological safety. This study aimed to evaluate the anti-inflammatory effects and acute and subacute toxicity of orally administered DXY powder in mouse and rat models, respectively. The anti-inflammatory effect was evaluated by comparing the intensity of edema and granuloma to that induced by extract (positive control). The toxicological effects were evaluated by assessing clinical signs, body weight, food intake, water consumption, blood biochemical and hematological parameters, organ coefficients, and organ histopathology in the treated rats. Oral administration of DXY powder in once-daily doses of 0.32 and 0.64 g/kg/day for 7 consecutive days effectively prevented xylene-induced ear edema in mice compared with the normal control group. In the acute and subacute toxicity evaluations, no clinical signs of toxicity, mortality, and no adverse effects on the target organs were observed. No significant pathological changes in any organs or tissues were observed at a daily dose of 3.0 g/kg for 6 consecutive months. However, significant pathological changes were observed in heart, liver, kidney, and intestinal tissues from the DXY powder-treated rats following daily doses of 12.0 and 48.0 g/kg for 6 consecutive months. Further studies are needed to determine whether these effects are toxic and reversible. The effective anti-inflammatory dose is far below the toxicity threshold. This study lays the foundation for the safety of DXY powder to justify its use as feed additives in veterinary clinical use.
丹参酮（DXY）是一种广泛使用的中药成分，可作为减少兽用抗生素使用的替代品。但很少有研究探讨其抗炎作用和毒理学安全性。本研究旨在分别评估口服DXY粉末在小鼠和大鼠模型中的抗炎作用以及急性和亚急性毒性。通过比较水肿和肉芽肿的强度与提取物（阳性对照）诱导的强度来评估抗炎作用。通过评估治疗大鼠的临床症状、体重、食物摄入量、饮水量、血液生化和血液学参数、器官系数以及器官组织病理学来评估毒理学效应。与正常对照组相比，连续7天每天口服0.32和0.64 g/kg/天剂量的DXY粉末可有效预防小鼠二甲苯诱导的耳水肿。在急性和亚急性毒性评估中，未观察到毒性的临床症状、死亡率以及对靶器官的不利影响。连续6个月每天给予3.0 g/kg的剂量时，未观察到任何器官或组织有明显的病理变化。然而，连续6个月每天给予12.0和48.0 g/kg剂量的DXY粉末后，在治疗大鼠的心脏、肝脏、肾脏和肠道组织中观察到明显的病理变化。需要进一步研究以确定这些影响是否具有毒性以及是否可逆。有效的抗炎剂量远低于毒性阈值。本研究为DXY粉末的安全性奠定了基础，以证明其在兽医临床用作饲料添加剂的合理性。
