Le Nhan Trong, Van Nguyen Hung, Le Gon Quang, Hoang Trang Huyen Xuan, Nguyen Khan Viet, Ho Duc Viet, Nguyen Ha Thu Thi, Nguyen Hoai Thi
Faculty of Pharmacy, Hue University of Medicine and Pharmacy, Hue University, Hue City, Vietnam.
Faculty of Traditional Medicine, Hanoi Medical University, Ha Noi City, Vietnam.
Anal Methods. 2025 Feb 13;17(7):1567-1578. doi: 10.1039/d4ay01885k.
Triterpenoids are known for their promising biological activities, and there is a growing focus on green extraction methods for these compounds. In this study, ultrasound-assisted deep eutectic solvents were employed to extract triterpenoids from persimmon leaves, with choline chloride-lactic acid identified as an effective green solvent. The extraction conditions were carefully optimized using response surface methodology, resulting in an extraction efficiency of 12.41%, which is 1.54 times higher than that achieved with conventional organic solvents such as methanol. The triterpenoids were then successfully recovered using a solid-liquid extraction method based on AB-8 macroporous resins, achieving a 94.01% recovery rate with a final product triterpenoid content of 81.87%. These findings provide a strong foundation for the sustainable extraction of triterpenoids and other valuable compounds from biomass.
三萜类化合物以其具有前景的生物活性而闻名，并且对这些化合物的绿色提取方法的关注日益增加。在本研究中，采用超声辅助深共熔溶剂从柿叶中提取三萜类化合物，已确定氯化胆碱 - 乳酸为一种有效的绿色溶剂。使用响应面法仔细优化了提取条件，提取效率达到12.41%，这比使用甲醇等传统有机溶剂所达到的效率高1.54倍。然后基于AB - 8大孔树脂通过固液萃取法成功回收了三萜类化合物，回收率达到94.01%，最终产品的三萜类化合物含量为81.87%。这些发现为从生物质中可持续提取三萜类化合物和其他有价值的化合物奠定了坚实基础。
