Silva Romério R S, Santos Maria H C, Santos Ana L E, Freitas Cleverson D T, Carneiro Rômulo F, Nagano Celso S, Mesquita Felipe P, Souza Pedro F N, Teixeira Claudener S
Department of Biochemistry and Molecular Biology, Federal University of Ceará, Fortaleza 60451-970, CE, Brazil.
Medical School, Federal University of Cariri, Barbalha, Ceará 63048-080, Brazil.
ACS Omega. 2025 Aug 29;10(35):40046-40055. doi: 10.1021/acsomega.5c04868. eCollection 2025 Sep 9.
is an important opportunistic fungal pathogen, and its resistance to conventional treatments poses a substantial challenge. Previous research by our group demonstrated that the anticandidal activity of seed lectin (DVL) involves multiple mechanisms of action. Our current objective is to analyze changes in the proteome of cells after treatment with the DVL lectin. The proteomic analysis corroborated the previously observed mechanisms with greater specificity, encompassing processes such as cell wall integrity, expression of transport proteins, proteins related to metabolism and energy, DNA repair proteins, and proteins related to defense and stress, and downregulated cell cycle proteins affecting cell viability. Our findings provide novel insights into response to DVL lectin, emphasizing the intricate cellular mechanisms underlying stress adaptation. These results provided new insight into the mechanisms of action of DVL against . They may facilitate the development of more effective and innovative antifungal therapies by providing a comprehensive understanding of fungal pathogenesis.
是一种重要的机会性真菌病原体，其对传统治疗的耐药性构成了重大挑战。我们小组之前的研究表明，种子凝集素（DVL）的抗念珠菌活性涉及多种作用机制。我们目前的目标是分析用DVL凝集素处理后细胞蛋白质组的变化。蛋白质组学分析以更高的特异性证实了先前观察到的机制，包括细胞壁完整性、转运蛋白表达、与代谢和能量相关的蛋白质、DNA修复蛋白以及与防御和应激相关的蛋白质等过程，并下调了影响细胞活力的细胞周期蛋白。我们的研究结果为对DVL凝集素的反应提供了新的见解，强调了应激适应背后复杂的细胞机制。这些结果为DVL对的作用机制提供了新的见解。它们可能通过全面了解真菌发病机制，促进更有效和创新的抗真菌疗法的开发。
