Matheson N K, Saini H S
Carbohydr Res. 1977 Aug;57:103-16. doi: 10.1016/s0008-6215(00)81924-2.
Extraction of germinating-lupin cocyledons, followed by ion-exchange and gel chromatography, gave two alpha-L-arabinofuranosidases and three beta-D-galactopyranosidases. Some fractions were further purified by using Concanavalin A-Sepharose. The changes in the activities of the enzymes during germination have been determined. Some kinetic and physical properties of these enzymes are described, and their role in the modification of cell-wall polysaccharides is discussed.
提取发芽羽扇豆的子叶，然后进行离子交换和凝胶色谱法，得到了两种α-L-阿拉伯呋喃糖苷酶和三种β-D-吡喃半乳糖苷酶。一些组分通过使用伴刀豆球蛋白A-琼脂糖进一步纯化。已测定了发芽过程中这些酶活性的变化。描述了这些酶的一些动力学和物理性质，并讨论了它们在细胞壁多糖修饰中的作用。
