对G(M1)神经节苷脂和乳糖基神经酰胺具有活性的纯化人肝脏酸性β-D-半乳糖苷酶。
Purified human liver acid beta-D-galactosidases possessing activity towards G(M1)-ganglioside and lactosylceramide.
作者信息
Miller A L, Frost R G, O'Brien J S
出版信息
Biochem J. 1977 Sep 1;165(3):591-4. doi: 10.1042/bj1650591.
Abstract
Our studies with purified human liver acid beta-D-galactosidases (EC 3.2.1.23) indicate that 4-methylumbelliferyl beta-D-galactosidase and G(M1)-ganglioside beta-D-galactosidase activities are identical with lactosylceramidase II activity. Evidence for this includes co-purification of all enzyme activities by affinity chromatography to yield a single band on polyacrylamide-gel electrophoresis and coincident elution from Sepharose 6B of all three enzyme activities.
摘要
我们对纯化的人肝脏酸性β-D-半乳糖苷酶（EC 3.2.1.23）的研究表明，4-甲基伞形酮基β-D-半乳糖苷酶和G(M1)-神经节苷脂β-D-半乳糖苷酶活性与乳糖神经酰胺酶II活性相同。支持这一结论的证据包括，通过亲和层析对所有酶活性进行共纯化，从而在聚丙烯酰胺凝胶电泳上产生单一谱带，以及所有三种酶活性从琼脂糖6B上同时洗脱下来。
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