Cao Xiaoyu, Zhang Yu, Xu Qianqian, Yan Hai
School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, China.
Front Microbiol. 2025 Aug 29;16:1624208. doi: 10.3389/fmicb.2025.1624208. eCollection 2025.
YZ01, a novel strain isolated from the feces of a healthy individual, demonstrated potent biodegradation of uric acid (54.13%) and nucleosides (41.21%). Phylogenomic analysis of 2,198 single-copy orthologous genes confirmed strain YZ01's taxonomic identity, while genome mining uncovered five circular antimicrobial peptide clusters. has been extensively utilized in the food industry for decades and is on the U.S. Food and Drug Administration's GRAS (Generally Recognized as Safe) list. Integrated genomic and phenotypic characterization revealed the probiotic properties and safety profile of YZ01, and proposed pathways for its biodegradation of uric acid and nucleosides. assays indicated that YZ01 exhibits excellent acid and bile salt tolerance as well as good adhesion, supported by the presence of stress-response genes, adhesion-associated surface proteins, and bile salt hydrolase genes. Antibiotic resistance genes and virulence factors were identified and confirmed to be safe through associated tests (antibiotic resistance, hemolytic assay, cytotoxicity assay and biogenic amine production). Furthermore, pilot-scale fermentation in a 100 L fermenter achieved high-density biomass production (6.12 × 10 CFU/mL) under optimized culture conditions, underscoring its industrial scalability. These findings highlight YZ01's bioactive properties, food-safe profile, industrial feasibility, and promising potential as a probiotic candidate for the development of functional foods aimed at improving hyperuricemia.
YZ01是从一名健康个体粪便中分离出的新型菌株，它对尿酸（54.13%）和核苷（41.21%）具有高效的生物降解能力。对2198个单拷贝直系同源基因进行系统基因组分析确定了YZ01菌株的分类身份，而基因组挖掘发现了5个环状抗菌肽簇。[具体物质]已在食品工业中广泛应用数十年，且在美国食品药品监督管理局的一般认为安全（GRAS）名单上。综合基因组和表型特征揭示了YZ01的益生菌特性和安全性概况，并提出了其对尿酸和核苷进行生物降解的途径。实验表明，YZ01表现出优异的耐酸和耐胆盐能力以及良好的黏附性，应激反应基因、黏附相关表面蛋白和胆盐水解酶基因的存在为此提供了支持。通过相关测试（抗生素抗性、溶血试验、细胞毒性试验和生物胺产生）鉴定并确认了抗生素抗性基因和毒力因子是安全的。此外，在100升发酵罐中进行的中试规模发酵在优化的培养条件下实现了高密度生物质生产（6.12×10 CFU/mL），突出了其工业可扩展性。这些发现突出了YZ01的生物活性特性、食品安全概况、工业可行性以及作为开发旨在改善高尿酸血症的功能性食品的益生菌候选物的广阔潜力。
