CM000152的基因组序列草案数据：从一名印度患者的脓液样本中分离出的菌株。
Draft Genome Sequence data of CM000152: A strain isolated from pus sample of an Indian patient.
作者信息
Sree B Sindhu, Bhattacharya Rajarshi, Mr Varshith, Ms Shrilaxmi, Choudhary Soumi, Lal Piyush Behari, Dastidar Ranita Ghosh, Mukhopadhyay Chiranjay, Dastidar Somasish Ghosh
机构信息
Centre for Molecular Neurosciences, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
Center for Emerging and Tropical Diseases, Kasturba Medical College, Manipal, Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.
出版信息
Data Brief. 2025 Aug 20;62:111991. doi: 10.1016/j.dib.2025.111991. eCollection 2025 Oct.
Here we report a draft genome sequence of strain CM000152, isolated from a melioidosis patient in India. This bacterium is a Gram-negative, saprophytic flagellate that can form biofilms. QIAamp® DNA Mini Kit (Qiagen, Chadstone, Victoria, Australia) was used to isolate genomic DNA from strain CM000152. It was further sequenced using the Illumina Hiseq 2500 platform (Illumina, Inc., San Diego, CA) to generate paired-end fragments (2 × 150 PE reads), producing 7146,272 reads at 284x coverage. We identified 246 contigs containing 300 base pairs with L50 of 36 and N50 of 67,418. CM000152 genome contains 7542,791 base pairs, with 67.71 % GC content. Gene prediction is performed using Prokka v.1.14.6. There are 7829 coding sequences in the sequenced DNA, followed by 74 genes coding tRNA and 2 coding rRNA. This project has been deposited under the NCBI Bioproject accession number PRJNA1190241.
在此，我们报告从印度一名类鼻疽患者分离出的CM000152菌株的基因组序列草图。这种细菌是革兰氏阴性、腐生鞭毛菌，能够形成生物膜。使用QIAamp® DNA Mini试剂盒（Qiagen公司，澳大利亚维多利亚州查德斯顿）从CM000152菌株中分离基因组DNA。使用Illumina Hiseq 2500平台（Illumina公司，加利福尼亚州圣地亚哥）对其进行进一步测序，以生成配对末端片段（2×150 PE读段），在284倍覆盖度下产生7146272条读段。我们鉴定出246个重叠群，其长度为300个碱基对，L50为36，N50为67418。CM000152基因组包含7542791个碱基对，GC含量为67.71%。使用Prokka v.1.14.6进行基因预测。测序DNA中有7829个编码序列，随后是74个编码tRNA的基因和2个编码rRNA的基因。该项目已存入NCBI生物项目登录号PRJNA1190241下。
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