Rijksen G, Schoop I, Staal G E
Clin Chim Acta. 1977 Oct 1;80(1):193-202. doi: 10.1016/0009-8981(77)90279-0.
The kinetic, electrophoretic and immunological properties of hexokinase from human erythrocytes have been studied in relation to cell age. No differences in kinetic behaviour between hexokinase partly purified from reticulocytes, 10% youngest cells, normal red cell population or from 10% oldest cells were observed. The stability of the enzyme preparations showed little differences; hexokinase from the 10% youngest cells was the most labile enzyme, followed respectively by the enzyme from reticulocytes, normal red cell population and the 10% oldest cells. The electrophoretic pattern of erythrocyte hexokinase changed during senescence. The hexokinase activity located in the second band from the anode is shifted to the third with increasing cell age. The molecular specific acitivity of the enzyme from the 10% youngest cells, the normal red cell population and the 10% oldest cells remains the same, while the molecular specific activity of hexokinase from reticulocytes was much lower.
已针对细胞年龄研究了人红细胞己糖激酶的动力学、电泳和免疫学特性。未观察到从网织红细胞（最年轻细胞的10%）、正常红细胞群体或最老细胞的10%中部分纯化的己糖激酶在动力学行为上存在差异。酶制剂的稳定性差异不大；最年轻细胞的10%中的己糖激酶是最不稳定的酶，其次分别是来自网织红细胞、正常红细胞群体和最老细胞的10%中的酶。衰老过程中红细胞己糖激酶的电泳图谱发生了变化。随着细胞年龄的增加，位于阳极第二条带的己糖激酶活性转移到第三条带。最年轻细胞的10%、正常红细胞群体和最老细胞的10%中的酶的分子比活性保持不变，而网织红细胞中己糖激酶的分子比活性则低得多。
