Kaplan J C, Beutler E
Science. 1968 Jan 12;159(3811):215-6. doi: 10.1126/science.159.3811.215.
The electrophoretic mobility of hexokinase from human erythrocytes and other tissues was studied with a new method that depends on the fluorescence of reduced nicotinamide-adenine dinucleotide phosphate for detecting enzyme activity on starch gel. The hexokinase of cord-blood erythrocytes has slightly different electrophoretic properties from that of adult red cells. Type I enzyme is split into type I(A) and type I(F); the latter is more intense in cord blood; in hemolyzates of adult blood, the activity of the two bands is usually about equal. No type II enzyme was found in cord blood. The double type I band was present in red cells from adult rabbits.
采用一种新方法研究了人红细胞及其他组织中己糖激酶的电泳迁移率，该方法依靠还原型烟酰胺腺嘌呤二核苷酸磷酸的荧光来检测淀粉凝胶上的酶活性。脐血红细胞的己糖激酶电泳特性与成人红细胞略有不同。I型酶可分为I(A)型和I(F)型；后者在脐血中更强；在成人血液的溶血产物中，两条带的活性通常大致相等。脐血中未发现II型酶。成年兔的红细胞中存在I型双条带。
