Xu Jiazhi, Nong Dongxiao, Nong Xiaolin, Zhao Jun, Bai Rui, Tang Chan, Hang Jiayi
Department of Oral and Maxillofacial Surgery, College of Stomatology, Hospital of Stomatology, Guangxi Medical University, Nanning, Guangxi Province, China.
Department of Otorhinolaryngology-Head and Neck Surgery, First Affiliated Hospital of Guangxi Medical University, Guangxi Medical University, Nanning, Guangxi Province, China.
Medicine (Baltimore). 2025 Sep 12;104(37):e44217. doi: 10.1097/MD.0000000000044217.
This study investigates the alterations in the composition of the oral microbiome in individuals with oral squamous cell carcinoma (OSCC) and examine the relationship between the oral microbiome and immune-related variables in the tumor immunological microenvironment of the host. The expression levels of immune factors interleukin-2 (IL-2), interleukin-10 (IL-10), and interferon-γ (IFN-γ) in the peripheral blood of OSCC patients and healthy volunteers were investigated using the enzyme-linked immunosorbent test (ELISA). Saliva samples were obtained from both OSCC patients and healthy control participants. The bacterial 16SrRNA gene was then analyzed using high-throughput sequencing to compare the composition and quantity of oral flora between OSCC patients and healthy volunteers. The bacteria that exhibited notable differences were compared with each other. Analyzed the correlation between the systemic immunological state of the host. The serum levels of IL-2 and IFN-γ were significantly greater in the OSCC group compared to the healthy controls (HC) group (IL-2, P<.001, IFN-γ, P<.01). In contrast, the serum IL-10 expression level in the OSCC group was significantly lower than that in the HC group (P <.01). The bacterial genera that differ between the OSCC group and the HC group are associated with the host immunological markers. Peptostreptococcus exhibited a noteworthy positive correlation with IL-2, but Thermus demonstrated a negative correlation with IL-2. The presence of IL-10 was shown to be positively associated with the genera Oralobacter and negatively associated with the genus Eikenella. The genus Peptostreptococcus had a notable positive connection with IFN-γ and a negative correlation with unclassified Actinobacteria. Significant differences in immune factor expression and oral microbiota were found between OSCC patients and HC. Microbial dysbiosis, particularly involving Fusobacterium and Veillonella, may influence OSCC progression by modulating the immune microenvironment.
本研究调查口腔鳞状细胞癌（OSCC）患者口腔微生物群组成的变化，并探讨口腔微生物群与宿主肿瘤免疫微环境中免疫相关变量之间的关系。采用酶联免疫吸附试验（ELISA）检测OSCC患者和健康志愿者外周血中免疫因子白细胞介素-2（IL-2）、白细胞介素-10（IL-10）和干扰素-γ（IFN-γ）的表达水平。从OSCC患者和健康对照者中获取唾液样本。然后使用高通量测序分析细菌16SrRNA基因，以比较OSCC患者和健康志愿者之间口腔菌群的组成和数量。对表现出显著差异的细菌进行相互比较。分析宿主全身免疫状态之间的相关性。与健康对照组（HC）相比，OSCC组的血清IL-2和IFN-γ水平显著更高（IL-2，P<.001，IFN-γ，P<.01）。相比之下，OSCC组的血清IL-10表达水平显著低于HC组（P<.01）。OSCC组和HC组之间不同的细菌属与宿主免疫标志物相关。消化链球菌与IL-2呈显著正相关，而栖热菌与IL-2呈负相关。IL-10的存在与口腔杆菌属呈正相关，与艾肯菌属呈负相关。消化链球菌属与IFN-γ有显著正相关，与未分类放线菌属呈负相关。OSCC患者和HC之间在免疫因子表达和口腔微生物群方面存在显著差异。微生物失调，特别是涉及具核梭杆菌和韦荣球菌，可能通过调节免疫微环境影响OSCC的进展。
